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Jmjd3-mediated H3K27me3 dynamics orchestrate brown fat cell fate transition and regulate white fat plasticity (RNA-Seq)

ABSTRACT: We performed RNA-Seq for brwon fat, epididymal white fat and soleus muscle of mice to identify brown fat-selective, white fat-selective and common fat genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE56367 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA243057

REPOSITORIES: GEO

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Jmjd3-mediated H3K27me3 dynamics orchestrate brown fat cell fate transition and regulate white fat plasticity (RNA-Seq)

Project description:We performed RNA-Seq for brwon fat, epididymal white fat and soleus muscle of mice to identify brown fat-selective, white fat-selective and common fat genes. RNA-Seq for brown fat, white fat and soleus muscle of wild type C56BL6 mice.

2015-12-31 | E-GEOD-56367 | biostudies-arrayexpress

Jmjd3-mediated H3K27me3 dynamics orchestrate brown fat cell fate transition and regulate white fat plasticity

Project description:We performed ChIP-seq to chart genome-wide maps of H3K27me3 in brown preadipocytes and mature brown adipocytes. We observed a subset of brown fat-specific genes, but not common fat genes or white fat-specific genes, possess the H3K27me3 mark in preadipocytes, and this mark is erased in mature adipocytes.

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Jmjd3-mediated H3K27me3 dynamics orchestrate brown fat cell fate transition and regulate white fat plasticity

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Jmjd3-Mediated H3K27me3 Dynamics Orchestrate Brown Fat Development and Regulate White Fat Plasticity.

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Project description:Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT-specific long non-coding RNAs (lncRNAs), we used RNA-seq to reconstruct de novo transcriptomes of mouse brown, inguinal white, and epididymal white fat and identified ~1500 lncRNAs, including 127 BAT-restricted loci induced during differentiation and often targeted by key regulators PPARγ, C/EBPα and C/EBPβ. One of them, lnc-BATE1, is required for establishment and maintenance of BAT identity and thermogenic capacity. lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes, and is partially rescued by exogenous lnc-BATE1 with mutated siRNA-targeting sites, demonstrating a function in trans. We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our work provides an annotated catalog for the study of fat depot-selective lncRNAs, available online, and establishes lnc-BATE1 as a novel regulator of BAT development and physiology. Total RNA profiles of BAT, iWAT and eWAT samples were sequenced on the Illumina HiSeq2000 platform

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