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Characterizing the profiles of Ebf1 in mouse fat cells.

ABSTRACT: gene expression data from mouse adipocyte, with and without Ebf1 knock-down

ORGANISM(S): Mus musculus

PROVIDER: GSE58193 | GEO | 2014/06/04

SECONDARY ACCESSION(S): PRJNA251567

REPOSITORIES: GEO

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Characterizing the profiles of Ebf1 in mouse fat cells.

Project description:gene expression data from mouse adipocyte, with and without Ebf1 knock-down To ascertain the functional targets of Ebf1 in adipocytes, we depleted Ebf1 from mature 3T3-L1 cells by transducing them with lentivirus harboring one of two different Ebf1-specific hairpins or a scrambled control. We then performed expression profiling using Affymetrix arrays with triplicate samples for each hairpin.

2014-06-04 | E-GEOD-58193 | biostudies-arrayexpress

Characterizing the profiles of Ebf1 in mouse fat cells (ChIP-seq)

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Characterizing the profiles of Ebf1 in mouse fat cells (ChIP-seq)

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Project description:NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogenesis, early B-cell factor 1 (EBF1), commitment, differentiation, NIH-3T3, pparg

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