Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spatiotemporal regulation of GLI target genes in the mammalian limb bud

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE58222 | GEO | 2015/07/01

SECONDARY ACCESSION(S): PRJNA253126

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Spatiotemporal regulation of GLI target genes in the mammalian limb bud

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-07-01 | E-GEOD-58222 | biostudies-arrayexpress

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.

Project description:GLI proteins convert Sonic hedgehog (Shh) signaling into a transcriptional output in a tissue-specific fashion. The Shh pathway has been extensively studied in the limb bud, where it helps regulate growth through a SHH-FGF feedback loop. However, the transcriptional response is still poorly understood. We addressed this by determining the gene expression patterns of approximately 200 candidate GLI-target genes and identified three discrete SHH-responsive expression domains. GLI-target genes expressed in the three domains are predominately regulated by derepression of GLI3 but have different temporal requirements for SHH. The GLI binding regions associated with these genes harbor both distinct and common DNA motifs. Given the potential for interaction between the SHH and FGF pathways, we also measured the response of GLI-target genes to inhibition of FGF signaling and found the majority were either unaffected or upregulated. These results provide the first characterization of the spatiotemporal response of a large group of GLI-target genes and lay the foundation for a systems-level understanding of the gene regulatory networks underlying SHH-mediated limb patterning.

| S-EPMC4587286 | biostudies-literature

Spatiotemporal regulation of GLI target genes in the mammalian limb bud

Project description:Spatiotemporal regulation of GLI target genes in the mammalian limb bud

| PRJNA253126 | ENA

Anisotropic stress orients remodelling of mammalian limb bud ectoderm.

Project description:The physical forces that drive morphogenesis are not well characterized in vivo, especially among vertebrates. In the early limb bud, dorsal and ventral ectoderm converge to form the apical ectodermal ridge (AER), although the underlying mechanisms are unclear. By live imaging mouse embryos, we show that prospective AER progenitors intercalate at the dorsoventral boundary and that ectoderm remodels by concomitant cell division and neighbour exchange. Mesodermal expansion and ectodermal tension together generate a dorsoventrally biased stress pattern that orients ectodermal remodelling. Polarized distribution of cortical actin reflects this stress pattern in a ?-catenin- and Fgfr2-dependent manner. Intercalation of AER progenitors generates a tensile gradient that reorients resolution of multicellular rosettes on adjacent surfaces, a process facilitated by ?-catenin-dependent attachment of cortex to membrane. Therefore, feedback between tissue stress pattern and cell intercalations remodels mammalian ectoderm.

| S-EPMC4955842 | biostudies-literature

GLI transcriptional repression regulates enhancer activity and chromatin accessibility for Hedgehog target genes

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-01-21 | GSE108880 | GEO

A Gli silencer is required for robust repression of gremlin in the vertebrate limb bud.

Project description:The transcriptional response to the Hedgehog (Hh) pathway is mediated by Gli proteins, which function as context-dependent transcriptional activators or repressors. However, the mechanism by which Gli proteins regulate their target genes is poorly understood. Here, we have performed the first genetic characterization of a Gli-dependent cis-regulatory module (CRM), focusing on its regulation of Grem1 in the mouse limb bud. The CRM, termed GRE1 (Gli responsive element 1), can act as both an enhancer and a silencer. The enhancer activity requires sustained Hh signaling. As a Gli-dependent silencer, GRE1 prevents ectopic transcription of Grem1 driven through additional CRMs. In doing so, GRE1 works with additional GREs to robustly regulate Grem1. We suggest that multiple Gli CRMs may be a general mechanism for mediating a robust transcriptional response to the Hh pathway.

| S-EPMC3994773 | biostudies-literature

Sp8 regulatory function in the limb bud ectoderm

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-01 | GSE144733 | GEO

Runx2-target molecules in limb bud cells

Project description:To identify novel molecules involved in osteoblastogenesis and bone formation, RNA-seq analyses was performed using limb bud cells infected with Venus control, Runx2 or Bmp2.

2021-08-31 | GSE166982 | GEO

Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-31 | GSE192486 | GEO

Generation of Human Expandable Progenitors with Limb-Bud Features via Chemically Induced Dedifferentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-09-01 | GSE243931 | GEO