Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of Wild-Type FVB/NJ Mouse Primary Cardiomyocyte and Cardiac Nonmyocyte Polyadenylated RNA and small RNA

ABSTRACT: Cells from three adult, wild-type, FVB hearts were separated into cardiomyocyte and nonmyocyte fractions using Langendorff perfusion, collagenase digestion and gravity filtration. Total RNA was prepared immediately from myocytes, while nonmyocytes were passaged twice to yield a culture from which total RNA was prepared.

ORGANISM(S): Mus musculus

PROVIDER: GSE58453 | GEO | 2014/06/14

SECONDARY ACCESSION(S): PRJNA252668

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Next Generation Sequencing of Wild-Type FVB/NJ Mouse Primary Cardiomyocyte and Cardiac Fibroblast Polyadenylated RNA and small RNA

Project description:Cells from three adult, wild-type, FVB hearts were separated into cardiomyocyte and nonmyocyte fractions using Langendorff perfusion, collagenase digestion and gravity filtration; total RNA was prepared immediately from myocytes, while nonmyocytes were passaged twice to yield a culture of largely fibroblasts from which total RNA was prepared. 6 cardiac polyadenylated RNA (mRNA and lncRNA) and small RNA (microRNA) profiles of isolated cardiomyocytes and fibroblasts from 12-wk FVB/NJ mouse hearts were generated on Illumina HiSeq 2000 instruments.

2014-06-14 | E-GEOD-58453 | biostudies-arrayexpress

(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 spleen expression data

2008-11-30 | GSE13225 | GEO

(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 lung expression data

2008-11-30 | GSE13224 | GEO

(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 Thymus expression data

2008-11-30 | GSE13227 | GEO

(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 blood expression data

2008-11-30 | GSE13222 | GEO

Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA

Project description:Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages. These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 40 cardiac polyA+-RNA profiles of 4- to 16 week-old FVB/NJ wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.

2014-03-12 | E-GEOD-55790 | biostudies-arrayexpress

(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 bone marrow expression data

2008-11-30 | GSE13223 | GEO

Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA After 1 Week Pressure Overload

Project description:Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. 5 female mice were subjected to a sham operation, and 5 female mice were subjected to transverse aortic constriction (TAC). After 1 week, hearts were harvested and polyadenylated RNAs were profiled. Analyzed data have been published in Hu et al., Proc Natl Acad Sci USA. 2012;109(48):19864-9, PMID: 23150554 10 cardiac polyA+-RNA profiles of 9 week-old FVB/NJ wild type (WT) mice (5 female sham, 5 female TAC) were generated on Illumina HiSeq 2000 instruments.

2014-04-18 | E-GEOD-56890 | biostudies-arrayexpress

Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA

2014-03-12 | GSE55790 | GEO

Transcription profiling of mouse AKR/J x FVB/NJ F1 versus DBA/2J x FVB F1 lung

Project description:F1 hybrids from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses display a 20-fold difference in mammary tumor metastatic capacity, due to differences in inherited polymorphisms. Expression studies were performed to determine whether polymorphism-driven gene expression signatures predictive of outcome could be generated from normal tissues Experiment Overall Design: Lung from adult F1 animals from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses was collected and arrayed on Affymetrics chip to identify basal differences in gene expression between the different genotypes

2009-01-13 | E-GEOD-13224 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data