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The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression

ABSTRACT: Gene expression and splicing switches upon RBM4 overexpression

ORGANISM(S): Homo sapiens

PROVIDER: GSE58594 | GEO | 2014/09/18

SECONDARY ACCESSION(S): PRJNA252956

REPOSITORIES: GEO

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The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression

Project description:Gene expression and splicing switches upon RBM4 overexpression 2 Samples

2014-09-18 | E-GEOD-58594 | biostudies-arrayexpress

The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.

Project description:Splicing dysregulation is one of the molecular hallmarks of cancer. However, the underlying molecular mechanisms remain poorly defined. Here we report that the splicing factor RBM4 suppresses proliferation and migration of various cancer cells by specifically controlling cancer-related splicing. Particularly, RBM4 regulates Bcl-x splicing to induce apoptosis, and coexpression of Bcl-xL partially reverses the RBM4-mediated tumor suppression. Moreover, RBM4 antagonizes an oncogenic splicing factor, SRSF1, to inhibit mTOR activation. Strikingly, RBM4 expression is decreased dramatically in cancer patients, and the RBM4 level correlates positively with improved survival. In addition to providing mechanistic insights of cancer-related splicing dysregulation, this study establishes RBM4 as a tumor suppressor with therapeutic potential and clinical values as a prognostic factor.

| S-EPMC4159621 | biostudies-literature

RBM4 regulates esophageal cancer progression

Project description:In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on KYSE150_shRBM4 and KYSE150_ctl which based on KYSE150 cells

2023-12-01 | GSE193919 | GEO

RBM4 regulates lung cancer progression

Project description:In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on A549_shRBM4 and A549_ctl which based on A549 cells , H1299_shRBM4 and H1299_ctl which based on H1299 cells

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RBM4 regulates senescence

Project description:RNA-seq was performed on A549 and H1299 cells that stably knocking down RBM4 or control, in order to profile the gene expression change that were regulated by RBM4

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PAR-CLIP of RBM4 and HIF-2a

Project description:To identify which mRNAs bind to RBM4/HIF-2a Two PAR-CLIPs were performed: One of an RBM4 immunoprecipitation, and the other of a HIF-2a immunoprecipitation and excising the associated RBM4 band.

2012-05-06 | E-GEOD-36247 | biostudies-arrayexpress

Exon arrays from HeLa cells transfected with RBM4-specific siRNA

Project description:RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10M-bM-^@M-^S15% of WilmsM-bM-^@M-^Y tumours and more recently in leukemya, is considered to be a tumour suppressor. RBM4, a RBP whose splicing effect is inhibited by the +KTS isoform of the tumour-suppressor/activator WT1, exhibits altered expression in different tumours, and may be essential for proliferation. Moreover, RBM4 binds to a number of RNAs of genes involved in acute myeloid leukaemia and cell cycle control. These results suggest that RBM4 may be involved in alternative splicing, tumorigenesis and particularly leukaemogenesis. To determine the endogenous transcripts that are targeted by RBM4 at the genome-wide level, we decided to perform exon microarray studies. RBM4-specific siRNA has been used for knockdown of RBM4 in HeLa cells. RNA was extracted using a Qiagen RNA extraction kit from untransfected, mock and siRNA-transfected HeLa cells and quality of RNA for microarray analysis was checked using a Bioanalyzer 2100 (Agilent Technologies). Experiments were run in triplicates.

2012-07-14 | E-GEOD-32933 | biostudies-arrayexpress

Exon arrays from HeLa cells transfected with RBM4-specific siRNA

Project description:RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10–15% of Wilms’ tumours and more recently in leukemya, is considered to be a tumour suppressor. RBM4, a RBP whose splicing effect is inhibited by the +KTS isoform of the tumour-suppressor/activator WT1, exhibits altered expression in different tumours, and may be essential for proliferation. Moreover, RBM4 binds to a number of RNAs of genes involved in acute myeloid leukaemia and cell cycle control. These results suggest that RBM4 may be involved in alternative splicing, tumorigenesis and particularly leukaemogenesis. To determine the endogenous transcripts that are targeted by RBM4 at the genome-wide level, we decided to perform exon microarray studies.

2012-07-15 | GSE32933 | GEO

PAR-CLIP of RBM4 and HIF-2a

Project description:To identify which mRNAs bind to RBM4/HIF-2a

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The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration

Project description:DAZAP1 was depleted in culterd HEK 293T cells using shRNA and the resulting poly A RNA were isolated c-DNA library constructed and paired end sequenced on illumina Hi-seq 2000 platform the data was compared to a control shRNA depleted cell Gene expression and splicing switches upon DAZAP1 knockdown

2013-12-25 | E-GEOD-52745 | biostudies-arrayexpress

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