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Identification of iron regulated genes.

ABSTRACT: Identification of transcriptional changes of Ustilago maydis wild type strain FB1 grown in axenic culture in complete medium containing glucose without or with the addition of 10 µM FeSO4. Keywords: gene induction

ORGANISM(S): Mycosarcoma maydis

PROVIDER: GSE6037 | GEO | 2007/01/01

SECONDARY ACCESSION(S): PRJNA97765

REPOSITORIES: GEO

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Identification of iron regulated genes.

Project description:Identification of transcriptional changes of Ustilago maydis wild type strain FB1 grown in axenic culture in complete medium containing glucose without or with the addition of 10 ÂµM FeSO4. Keywords: gene induction Strain FB1 (wt) was grown in CM glucose medium in the presence or absence of 10 ÂµM FeSO4.

2010-06-12 | E-GEOD-6037 | biostudies-arrayexpress

Identification of genes regulated by the iron dependent transcription factor Urbs1.

Project description:Urbs1 of Ustilago maydis is a transcription factor that has been shown to repress its target genes (e.g. sid1, sid2) in the presence of iron. To identify additional genes regulated by Urbs1, we compared transcriptional profiles of the Ustilago maydis wild type strain FB1 grown in complete medium containing glucose with the addition of 10 µM FeSO4 to the Ustilago maydis urbs1 deletion mutant BW12 grown under the same conditions. Keywords: gene induction

2007-01-01 | GSE6038 | GEO

Identification of genes regulated by the iron dependent transcription factor Urbs1.

Project description:Urbs1 of Ustilago maydis is a transcription factor that has been shown to repress its target genes (e.g. sid1, sid2) in the presence of iron. To identify additional genes regulated by Urbs1, we compared transcriptional profiles of the Ustilago maydis wild type strain FB1 grown in complete medium containing glucose with the addition of 10 ÂµM FeSO4 to the Ustilago maydis urbs1 deletion mutant BW12 grown under the same conditions. Keywords: gene induction Strains FB1 (control) and BW12 (urbs1 deletion mutant) were grown in CM glucose in the presence of iron.

2010-06-12 | E-GEOD-6038 | biostudies-arrayexpress

Gene expression during biotrophic growth

Project description:Comparison between Ustilago maydis cells grown in axenic culture and U. maydis cells from plant tumors. Keywords: developmental stages U. maydis FB1 cells were grown in axenic culture; corn plants were infected with a mixture of strains FB1 and FB2, and tumors were harvested 13 days post infection. SAmples were taken from two biological replicates each.

2010-06-20 | E-GEOD-5804 | biostudies-arrayexpress

Identification of Adr1 regulated genes

Project description:Adr1 of Ustilago maydis is a protein kinase that is activated after separation from the regulatory subunit mediated by high cAMP levels. A copy of the gene under the control of the arabinose-inducible crg1 promotor was introduced into the Cbx locus of the wild type strain FB1 creating strain HE140. To monitor the transcriptional changes upon adr1 induction by arabinose, we conducted a time course experiment comparing the wild type transcriptional response before and 75 or 180 min after medium shift to complete medium containing arabinose, as well as the response of the mutant HE140 before and 75 or 180 min after induction of the additional copy of adr1 by arabinose. Keywords: gene induction

2007-01-01 | GSE6039 | GEO

Identification of Adr1 regulated genes

2010-06-12 | E-GEOD-6039 | biostudies-arrayexpress

Gene expression during biotrophic growth

Project description:Comparison between Ustilago maydis cells grown in axenic culture and U. maydis cells from plant tumors. Keywords: developmental stages

2008-09-01 | GSE5804 | GEO

Transcriptome-based comparison of the infection response in maize silks between Fusarium graminearum and Ustilago maydis

Project description:In this study, RNA-seq based comparative transcriptome analysis was used to study the response between Fusarium graminearum and Ustilago maydis to different growth conditions. RNA-seq libraries were generated from fungal filaments growing in culture (complete medium) and from infected maize silk. This data set contains the data for the Fusarium graminearum and Ustilago maydis medium growth condition.

2019-03-17 | E-MTAB-4562 | biostudies-arrayexpress

Ustilago maydis rak1 mediated gene expression during exponential growth

Project description:In mammalian cells RACK1 serves as a scaffold protein that has a role in integrating inputs from different signaling pathways and affects translation through association with ribosomes. U. maydis contains a seven-WD40 repeat motif protein designated Rak1, which shows 68% identity to RACK1 and is homologous to Asc1p of Saccharomyces cerevisiae. The asc1 mutant could be complemented by introduction of U. maydis rak1. The deletion of rak1 affected cell growth, cell wall integrity and specifically attenuated cell fusion. This latter defect was caused by reduced expression of prf1 encoding the regulator for pheromone and pheromone-receptor genes. Rak1 interacts with a variety of ribosomal proteins and microarray analysis revealed that the deletion of rak1 led to severely reduced expression of rop1, an activator prf1. The constitutive expression of rop1 could rescue the defect of mfa1 expression as well as conjugation tube formation in response to pheromone induction in the rak1 mutant. Moreover, a solopathogenic rak1 mutant failed to respond to plant derived stimuli, resulting in attenuated filamentation and pathogenicity. This could be partially rescued by constitutive expression of the b heterodimer. These data suggest that rak1 is a regulator of rop1 expression with additional roles after cell fusion. Ustilago maydis strains FB1 and FB1?rak1 were grown on a rotary shaker (200 rpm) in liquid Ustilago complete medium with 1% glucose at 28°C to an OD600 of 0.5. The experiment was performed using three independent biological replicates per strain.

2011-07-05 | E-GEOD-30382 | biostudies-arrayexpress

Time series transcriptional profiling of Ustilago maydis SG200 infecting Zea mays.

Project description:Goals: Comparing the infection between Ustilago maydis SG200 with the wild-type strain FB1xFB2 previously published Methods: Comparative RNASeq analysis between U. maydis SG200 and U. maydis FB1xFB2 at three timepoints (axenic, 2dpi, 12dpi) Results: The RNASeq analysis in SG200 identifies differences in gene expression with FB1xFB2. These differences could be the result of a unequal contribution of each nuclei to transcription. Further analysis identified a set of differentially transcribed genes.

2022-12-24 | GSE122788 | GEO

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