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Expression microarray analysis of HaCaT epidermal keratinocyte treated with AdcaRAS/dnRAS, p53 siRNA, and/or TGF-beta

ABSTRACT: We demonstrate that activation of Ras and TGF-β pathways strengthens the binding of p63 to its genomic sites and modulates the expression of p63 target genes. Our findings support a model of mutual inhibition between ΔNp63α, DUSP6 and mutant p53 downstream of oncogenic Ras and TGF-β.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60813 | GEO | 2016/08/12

SECONDARY ACCESSION(S): PRJNA259608

REPOSITORIES: GEO

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p63 binding regions in HaCaT epidermal keratinocyte infected with caRAS/dnRAS and treated with TGF-beta

Project description:We evaluated the effect of caRAS/dnRAS expression and TGF-beta on p63 binding in HaCaT cells.

2016-08-12 | GSE60812 | GEO

Profiling of genes regulated by TGF-beta in immortalized skin keratinocyte cells(HaCaT).

Project description:TGF-beta is one of the key cytokines implicated in various disease processes including cancer. TGF-beta inhibits growth and promotes apoptosis in normal epithelial cells and in contrast, acts as a pro-tumour cytokine by promoting tumour angiogenesis, immune-escape and metastasis. The effect of TGF-beta on the regulation of gene expression is cell-type specific. In order to identify TGF-beta regulated genes in different cell-types, we followed the expression profiling approach. Keywords: cell type specific response to TGF-beta

2007-05-01 | GSE7661 | GEO

Colon cancer stem cell over-expressing ΔNp63α

Project description:p63, a member of p53 family, is transcribed in different variants, containing (TA) or lacking (ΔN) the N-terminal transactivation domain. Although the proteins of p53 family share high sequence and structural similarities, distinct functions for p63 are emerging. Here we provided a quantitative proteomic analysis by stable isotope dimethyl labeling of colon cancer stem cells over-expressing ΔNp63α in order to investigate the cellular pathways modulated by this p63 isoform.

2014-03-17 | PXD000769 | Pride

Expression data from p63 siRNA in squamous cell lines

Project description:p63, a homologue of the tumor suppressor p53, is critical for the development and maintenance of squamous epithelia. p63 is specifically expressed in the basal layers of stratified epithelial tissues, and is considered to be a specific marker for cells of this type. The role of p63 in tumorigenesis remains poorly defined. Numerous studies have highlighted the oncogenic potential of the predominant p63 isoform, ΔNp63α; however, data suggests that other p63 proteins can act as tumor suppressors or alter the metastatic potential of tumors. ΔNp63α can act as a transcriptional repressor, but the link between the transcriptional functions of p63 and its biological role is still unclear. In this study, we used a loss-of-function approach to investigate the transcriptional programs controlled by p63. Keywords: cell line panel

2006-06-06 | GSE4975 | GEO

Transcription profiling of rat intestinal epithelial cells infected with Ras and treated with TGF-beta-1

Project description:RIE (rat intestinal epithelial) cells treated with 2 ng/ml of TGF-beta-1 for 1 hr were compared to control cells. The experiment was performed under identical conditions four times: RIE vs RIE - TGF-beta #1-#4. RIE-Ras cells were established by stable transfection of the parental cells with pSV2-H-Ras(12V) which contain human sequences encoding the constitutively active H-Ras(12V) protein. RIE-Ras cells treated with 2 ng/ml of TGF-beta-1 for 1 hr were compared to control cells. The experiment was performed four times under identical conditions (RIE-Ras vs RIE-RAS TGF-beta #1-#4).

2008-06-11 | E-GEOD-2015 | biostudies-arrayexpress

Intestinal epithelial cells transfected with Ras and treated with TGF-beta-1

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T-bet deficiency and Hic1 induction override TGF-b-dependency in the formation of CD103+ intestine-resident memory CD8+ T cells

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microRNA expression profile of MCF7 cells with retroviral inducible expression of ΔNp63α [miRNA]

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mRNA expression profile of MCF7 cells with retroviral inducible expression of ΔNp63α [total RNA]

2015-12-28 | GSE64953 | GEO

TGF-β and RREB1 coordinate EMT programs in NMuMG cells

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