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The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (ChvI-SyrA)

ABSTRACT: We characterized transcriptomes of strains containing a K214T mutation in chvI, with and without overexpression of syrA. Our work shows that overexpression of syrA suppresses many of the transcription changes observed in the chvI K214T mutant.

ORGANISM(S): Medicago truncatula Sinorhizobium meliloti

PROVIDER: GSE61521 | GEO | 2015/03/31

SECONDARY ACCESSION(S): PRJNA261377

REPOSITORIES: GEO

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The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (ChvI-SyrA)

Project description:We characterized transcriptomes of strains containing a K214T mutation in chvI, with and without overexpression of syrA. Our work shows that overexpression of syrA suppresses many of the transcription changes observed in the chvI K214T mutant. Gene expression profiling of a Sinorhizobium meliloti chvI K214 mutant overexpressing syrA was performed using custom Affymetrix GeneChips

2015-03-31 | E-GEOD-61521 | biostudies-arrayexpress

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrM)

Project description:We characterized transcriptomes of a strain overexpressing syrM. Our work shows that the syrM transcriptome shares similar gene expression changes to the syrA and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression.

2015-03-31 | GSE61525 | GEO

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)

Project description:We characterized transcriptomes of a strain overexpressing syrA. Our work shows that the syrA transcriptome shares similar gene expression changes to the syrM and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression.

2015-03-31 | GSE61524 | GEO

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)

2015-03-31 | E-GEOD-61524 | biostudies-arrayexpress

The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrM)

2015-03-31 | E-GEOD-61525 | biostudies-arrayexpress

Identifying the regulon of the Sinorhizobium meliloti JspA protease

Project description:Sinorhizobium meliloti establishes symbiotic relationship with compatible leguminous plants by inducing root nodule formation, colonizing such nodules, and fixing molecular nitrogen for the host in exchange for carbon compounds. This mutualistic process requires complex communication and tight regulation to allow yet constrain infection to specific tissues. Production of succinoglycan, or exopolysaccharide-I (EPS-I), enables S. meliloti to invade the root cortex via infection threads. A previous genetic screen identified jspA (SMc03872), encoding an extracytoplasmic protease, as a regulator of EPS-I production. To elucidate its molecular role, we performed transcriptome analyses of strains overexpressing wild-type or mutant alleles of jspA. We observed changes in gene expression suggesting that JspA contributes to symbiosis efficiency by modulating the critical ExoR-ExoS-ChvI signaling pathway.

2023-05-10 | GSE155833 | GEO

Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti

Project description:We performed transcriptional profiling with chvI gain-of-function (EC220) and reduced-function (EC69) strains. The chvI gain-of-function strain that we used contains a dominant gain-of-function chvI allele in addition to wild-type chvI. We identified genes that, relative to their expression level in the wild type, are both upregulated in the chvI gain-of-function strain and downregulated in the reduced-function strain or vice versa.

2018-04-07 | GSE112819 | GEO

Genome-wide identification of genes directly regulated by ChvI and a consensus sequence for ChvI binding in Sinorhizobium meliloti

Project description:We have used chromatin immunoprecipitation followed by microarray analysis (chIP-chip) to identify DNA regions bound by the ChvI protein in Sinorhizobium meliloti cells. We then used quantitative PCR with chvI mutant strains to test the ChvI-dependent expression of genes downstream of the ChvI-bound DNA regions.

2019-04-01 | GSE112818 | GEO