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The transcriptome of Legionella pneumophila-infected human monocyte-derived macrophages

ABSTRACT: Analysis of the human monocyte-derived macrophage (hMDM) transcriptional response to L. pneumophila infection at 8 hours post-infection We used microarrays to probe the hMDM transcriptional response to wild type L. pneumophila AA100 infection and its isogenic ankB and T2SS mutants. We identified up-regulation of innate immunity pathways and down-regulation of protein translation pathways

ORGANISM(S): Homo sapiens

PROVIDER: GSE61535 | GEO | 2014/09/19

SECONDARY ACCESSION(S): PRJNA261427

REPOSITORIES: GEO

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The transcriptome of Legionella pneumophila-infected human monocyte-derived macrophages

Project description:Analysis of the human monocyte-derived macrophage (hMDM) transcriptional response to L. pneumophila infection at 8 hours post-infection We used microarrays to probe the hMDM transcriptional response to wild type L. pneumophila AA100 infection and its isogenic ankB and T2SS mutants. We identified up-regulation of innate immunity pathways and down-regulation of protein translation pathways hMDMs were isolated from healthy donors and infected with L. pneumophila AA100 and its isogenic ankB and T2SS mutants for 8 h prior to isolation of total RNA and hybridization to Affymetrix microarrays.

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