Changes in global gene expression in SIN1 knock-out murine epithelial fibroblasts
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ABSTRACT: RNA from wt and SIN1 knock-out MEF cell lines were compared Using affymetrix murine gene chip, the effects of SIN1 ablation on global gene transcription were assessed
Project description:RNA from wt and SIN1 knock-out MEF cell lines were compared Using affymetrix murine gene chip, the effects of SIN1 ablation on global gene transcription were assessed RNA from wild type and SIN1 knock out cells was purified and differences in gene expression between the two sets. MEFs were derived from C57BL/6 (Jacinto et. al. Cell. 2006 Oct 6;127(1):125-37. Epub 2006 Sep 7
Project description:Comparison of responses of mouse wild-type, p53 Ser389 phosphorylation site point mutant and full knock-out mutant MEF cells to UV-C-radiation in time
Project description:MEF WT, MEF DKO(Bax/Bak), MEF DKO(Bax/Bak) expressing rabbit Serca2a, MEF DKO(Bax/Bak) expressing Bak at the endoplasmic reticulum both control and treated with Noco 100 nM for 48h
Project description:the gene expression profiling results provide important information for the genes regulated by crosstalk between Shp2 and Pten mediated signal pathways Total RNA was extracted from CD71mid Ter119high erythroblasts isolated from the bone marrow of wide type, Shp2 knock-out, Pten knock-out and double knock-out mice