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Comprehensive role of Zfp217 in m6A methylation [ChIP-seq]

ABSTRACT: Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses.

ORGANISM(S): Mus musculus

PROVIDER: GSE65730 | GEO | 2015/10/30

SECONDARY ACCESSION(S): PRJNA274813

REPOSITORIES: GEO

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Comprehensive role of Zfp217 in m6A methylation [ChIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses. Examination of Zfp217 binding profiling by high throughput sequencing in mouse stem cells

2015-10-30 | E-GEOD-65730 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells. Examination of m6A levels from total RNA in control and Zfp217 shRNA infected mouse stem cells

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Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells.

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Comprehensive role of Zfp217 in m6A methylation [RIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217and Mettl3 as RNA-binding proteins. Identification and characterization of RNAs associated with Zfp217 and Mettl3 in mouse embryoinc stem cells

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Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal. RNA-seq was used to measure gene expression levels in luciferase control and Zfp217 short hairpin RNA (shRNA) infected mouse stem cells

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Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal.

2015-10-30 | GSE65731 | GEO

Comprehensive role of Zfp217 in m6A methylation [RNA-seq RA]

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Project description:Self-renewal circuitry in embryonic stem (ES) cells is increasingly defined. How the robust pluripotency programme is dissolved to enable fate transition is less appreciated. We developed a forward genetic approach using haploid ES cells. We created libraries of transposon integrations and screened for persistent self-renewal in differentiation permissive culture. This yielded multiple mutants in the FGF/Erk and GSK3/Tcf3 modules known to drive differentiation, and in epigenetic modifiers implicated in lineage commitment. We also identified and validated factors not previously considered. These include the conserved small zinc finger protein Zfp706. Loss of Zfp706 function severely delays the exit from self-renewal. To asses a potential function of Zfp706 in regulation of gene expression we compared transcription profiles between Zfp706 gene trap mutant ES cells and genetic revertants. Assessment of differentially expressed genes in Zfp706 gene trap mutants obtained in a haploid ES cell screen for effectors of ES cell differentiation vs. genetic revertants generated by Flp-e mediated excision of the gene trap cassette

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Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of Trophoblast Stem Cells

Project description:Esrrb is a transcription factor implicated in embryonic stem (ES) cell self-renewal, yet its knockout causes intrauterine lethality due to defects in trophoblast development. Here we show that in trophoblast stem (TS) cells, Esrrb is a downstream target of fibroblast growth factor (Fgf) signalling and is critical to drive TS cell self-renewal. In contrast to its occupancy of pluripotency-associated loci in ES cells, Esrrb sustains the stemness of TS cells by direct binding and regulation of TS cell-specific transcription factors including Elf5 and Eomes. To elucidate the mechanisms whereby Esrrb controls the expression of its targets, we characterized its TS cell-specific interactome by mass spectrometry. Unlike in ES cells, Esrrb interacts in TS cells with the histone demethylase Lsd1 and with the RNA Polymerase II-associated Integrator complex. Our findings provide new insights into both, the general and context-dependent wiring of transcription factor networks in stem cells by master transcription factors.

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