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Comprehensive role of Zfp217 in m6A methylation [RIP-seq]

ABSTRACT: Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217and Mettl3 as RNA-binding proteins.

ORGANISM(S): Mus musculus

PROVIDER: GSE65733 | GEO | 2015/10/30

SECONDARY ACCESSION(S): PRJNA274810

REPOSITORIES: GEO

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Comprehensive role of Zfp217 in m6A methylation [RIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217and Mettl3 as RNA-binding proteins. Identification and characterization of RNAs associated with Zfp217 and Mettl3 in mouse embryoinc stem cells

2015-10-30 | E-GEOD-65733 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [ChIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses. Examination of Zfp217 binding profiling by high throughput sequencing in mouse stem cells

2015-10-30 | E-GEOD-65730 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells. Examination of m6A levels from total RNA in control and Zfp217 shRNA infected mouse stem cells

2015-10-30 | E-GEOD-65734 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [ChIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses.

2015-10-30 | GSE65730 | GEO

Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells.

2015-10-30 | GSE65734 | GEO

Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal. RNA-seq was used to measure gene expression levels in luciferase control and Zfp217 short hairpin RNA (shRNA) infected mouse stem cells

2015-10-30 | E-GEOD-65731 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal.

2015-10-30 | GSE65731 | GEO

Comprehensive role of Zfp217 in m6A methylation [RNA-seq RA]

Project description:Mouse stem cells were differenciated with retinoic acid and cells were harvested at the indicated time points. RNA-seq was used to measure gene expression levels in retinoic acid-differentiated mouse stem cells

2015-10-30 | E-GEOD-65732 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-10-30 | E-GEOD-65735 | biostudies-arrayexpress

m6A RNA Methylation is Critical for Adequate Exit from Naïve Pluripotency and Execution of Mammalian Development (RNA-Seq)

Project description:In this study we identify Mettl3, an m6A RNA modification writer, as a critical regulator for terminating naïve pluripotency and a positive maintainer of primed pluripotency in vitro and in vivo. Remarkably, Mettl3 knockout pre-implantation epiblasts and naïve ES cells, entirely lack m6A on coding mRNAs and are viable. Yet, they fail to adequately terminate the naïve pluripotent state, and subsequently undergo aberrant priming and early lineage commitment at the post-implantation stage. A comprehensive functional and genomic analysis involving profiling of m6A, RNA transcription and translation in Mettl3 wild-type and knockout pluripotent and differentiated cells, identified m6A as a critical determinant that destabilizes secondary naïve specific pluripotency genes Esrrb, Klf4 and Nanog, and restrains their transcript stability and translation efficiency. In summary, our findings provide for the first time evidence for a critical role for an mRNA epigenetic modification in early mammalian development in vivo, and identify a mechanism that functionally regulates mouse naïve and primed pluripotency in an opposing manner. polyA RNA-seq was measured in mouse embryonic stem cells (ESCs) and embroid bodies (EBs), each in WT and in Mettl3-KO cell lines. RNA-seq was measured also from WT mouse embronic fibroblasts (MEF). 3 biological replicates are available from ESCs and 2 from EBs. Replicate C in ESCs was measured alongside protein levels (SILAC) and was used for the analysis of that assay.

2014-11-02 | E-GEOD-61997 | biostudies-arrayexpress

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