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Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

ABSTRACT: Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal.

ORGANISM(S): Mus musculus

PROVIDER: GSE65731 | GEO | 2015/10/30

SECONDARY ACCESSION(S): PRJNA274812

REPOSITORIES: GEO

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Comprehensive role of Zfp217 in m6A methylation [RNA-seq]

Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal. RNA-seq was used to measure gene expression levels in luciferase control and Zfp217 short hairpin RNA (shRNA) infected mouse stem cells

2015-10-30 | E-GEOD-65731 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells. Examination of m6A levels from total RNA in control and Zfp217 shRNA infected mouse stem cells

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Comprehensive role of Zfp217 in m6A methylation [ChIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses. Examination of Zfp217 binding profiling by high throughput sequencing in mouse stem cells

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Comprehensive role of Zfp217 in m6A methylation [RIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217and Mettl3 as RNA-binding proteins. Identification and characterization of RNAs associated with Zfp217 and Mettl3 in mouse embryoinc stem cells

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Comprehensive role of Zfp217 in m6A methylation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-10-30 | E-GEOD-65735 | biostudies-arrayexpress

Comprehensive role of Zfp217 in m6A methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-10-30 | GSE65735 | GEO

Comprehensive role of Zfp217 in m6A methylation [RNA-seq RA]

Project description:Mouse stem cells were differenciated with retinoic acid and cells were harvested at the indicated time points. RNA-seq was used to measure gene expression levels in retinoic acid-differentiated mouse stem cells

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