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Comparison of spheroid-based 3-dimensional culture to monolayer of larynx carcinoma cell line HLaC78.

ABSTRACT: In vitro proliferation and/or invasion tests (such as MTT assay, Boyden Chamber Assay, Scratch assay) using established and well-characterized cell lines can provide easy and reproducible tools to investigate anti-tumour or anti-invasion agents ex vivo. However, in vivo cells in a tissue context interact with neighbouring cells and extracellular matrix components. In 2-dimensional monolayer cultures cells tend to loose tissue-specific properties. 2-D culture models therefore seem unsuitable to mimic the behaviour of cells in a natural, 3-dimensional microenvironment, especially for testing of growth or invasion inhibiting substances. Spheroids on the other hand resemble in many aspects solid, non-vascularized tumour tissue.

ORGANISM(S): Homo sapiens

PROVIDER: GSE67495 | GEO | 2016/03/11

SECONDARY ACCESSION(S): PRJNA280082

REPOSITORIES: GEO

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Comparison of spheroid-based 3-dimensional culture to monolayer of larynx carcinoma cell line HLaC78.

Project description:In vitro proliferation and/or invasion tests (such as MTT assay, Boyden Chamber Assay, Scratch assay) using established and well-characterized cell lines can provide easy and reproducible tools to investigate anti-tumour or anti-invasion agents ex vivo. However, in vivo cells in a tissue context interact with neighbouring cells and extracellular matrix components. In 2-dimensional monolayer cultures cells tend to loose tissue-specific properties. 2-D culture models therefore seem unsuitable to mimic the behaviour of cells in a natural, 3-dimensional microenvironment, especially for testing of growth or invasion inhibiting substances. Spheroids on the other hand resemble in many aspects solid, non-vascularized tumour tissue. Within this study the larynx carcinoma cell line HLaC78 was used as a model for comparing overall gene expression in 2D monolayer culture with 72 hrs old HLaC78 multicellular spheroids, providing evidence for extensive restructuring of gene expression in the 3-dimensional context.

2016-03-11 | E-GEOD-67495 | biostudies-arrayexpress

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Migration and invasion of 5 glioblastoma cell lines

2014-09-05 | E-GEOD-53124 | biostudies-arrayexpress

Established glioma cell lines prove valuable models for tumour heterogeneity - mediated cellular response studies in vitro

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Gene differential expressions between cell subpopulations with high invasiveness (invaded) and low invasiveness (non-invaded) in human lung cancer H460 cell line

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