Dual RNA-seq – Host response to pinT*
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA281339 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1976391.fastq.gz | Fastqsanger.gz | |||
| SRR1976392.fastq.gz | Fastqsanger.gz | |||
| SRR1976393.fastq.gz | Fastqsanger.gz | |||
| SRR1976394.fastq.gz | Fastqsanger.gz | |||
| SRR1976395.fastq.gz | Fastqsanger.gz |
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Project description:Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including infection-specific alterations of an array of long noncoding RNAs. Our study provides a paradigm for a comprehensive RNA-based analysis of intracellular bacterial pathogens without their physical purification from a host and a new discovery route for hidden functions of pathogen genes.
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