Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Investigating GPR34 expression regulation using whole transcriptome sequencing of spleens and dendritic cells from wildtype and GPR34 knockout mice

ABSTRACT: Naive spleens as well as naive and LPS-treated dendritic cells from wildtype and GPR34-/- mice were sequenced to integrate expression profiles with protein interaction networks and find functional modules that are affected by GPR34

ORGANISM(S): Mus musculus

PROVIDER: GSE68155 | GEO | 2016/03/02

SECONDARY ACCESSION(S): PRJNA281932

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Investigating GPR34 expression regulation using whole transcriptome sequencing of spleens and dendritic cells from wildtype and GPR34 knockout mice

Project description:Naive spleens as well as naive and LPS-treated dendritic cells from wildtype and GPR34-/- mice were sequenced to integrate expression profiles with protein interaction networks and find functional modules that are affected by GPR34 Expression profiles of dendritic cells and whole spleens were generated using Illumina HiSeq 2500/ Illumina HiScan

2016-03-02 | E-GEOD-68155 | biostudies-arrayexpress

RNA-seq of chicken spleens after LPS injection

Project description:RNA-seq of chicken spleens after LPS injection

| PRJEB28060 | ENA

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs and spleens)

Project description:Here we compare gene expression profiles between lungs and spleens following LPS in vivo injection into Wild type, lincRNA-Cox2 knockout and lincRNA-Cox2 mutant mice The aim of the experiment is to determine the cis and trans functions for lincRNA-Cox2 following in vivo LPS challenge within the spleen and lung

2018-12-19 | GSE116354 | GEO

RNA-seq of chicken spleens after LPS injection

Project description:The acute phase response is poorly studied in chickens, but its examination can possibly supply valuable markers for the identification of subclinical disease and assessment of disease severity. We therefore injected ten six weeks-old Lohmann Selected Leghorn chickens i.v. with 10µg LPS per kg body weight (n=5) or the same volume of PBS as controls (n=5) and collected spleens after three hours. Spleens were kept in ice cold PBS and RNA was isolated immediately. After quality control of RNA (RIN>9.0), a cDNA library was prepared and submitted to Illumina high-throughput sequencing.

2019-01-18 | E-MTAB-7082 | biostudies-arrayexpress

Expression data from spleens of mice immunized using LPS vs MPL as adjuvant.

Project description:An unresolved issue in immunology is the extent to which inflammatory effects are needed for robust T cell responses. In this study, mice were immunized by iv injection using either high toxicity lipopolysaccharide (LPS) or low toxicity monophosphoryl lipid A (MPL) as adjuvant. Six hours after iv immunization, whole spleens were harvested and gene expression was measured in unfractionated splenic populations of cells. The analysis indicated that the low toxicity adjuvanticity of MPL was associated with TLR4-mediated signaling that was biased to the TRIF branch of TLR4, while LPS generated balanced MyD88 and TRIF-associated outcomes. Keywords: Treatment comparison

2007-06-09 | GSE7768 | GEO

LPS-stimulated wildtype and miR-146a KO murine C57BL/6 Bone Marrow-derived Dendritic Cells

Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 100 ng/mL LPS for 24h. Cells were derived from either wildtype or microRNA-146a knock-out mice. Total RNA was isolated from the cells following stimulation.

2016-07-30 | E-MTAB-3539 | biostudies-arrayexpress

Transcription profiling of mouse dendritic cells from the spleens of Alymphoplasia or heterozygeous littlermates (wild type) to identify TNIK/NF-kappaB2 regulated gene products.

Project description:This study aims at identifying genes that are NIK/NF-kappaB2 responsive in murine dendritic cells matured in vivo. Experiment Overall Design: This study isolated dendritic cells from the spleens of Alymphoplasia or heterozygeous littlermates (wild type). Mice were eiter not treated as a reference or treated I.P. with 25 micrograms of LPS and 100 micrograms of agonistic anti-CD40 antibody. DCs were isolated 6 hours after treatment allowing in vivo maturation via both TLR and CD40 signals. DCs from 3 mice per group were pooled. Experiment was performed in triplicate.

2008-04-06 | E-GEOD-7219 | biostudies-arrayexpress

Lipopolysaccharide (LPS) effect on ficolin deficient spleen

Project description:Analysis of spleens from ficolin knockout and wildtype mice treated without and with LPS to induce inflammation. Ficolins are pattern recognition molecules that initiate the lectin pathway of complement. Results provide insigt into the molecular basis of the inflammatory response of ficolin knockout mice.

2017-02-22 | GSE95125 | GEO

Bulk RNA-Seq analysis of CD8+ T cells from treatment-naïve matched tumor-bearing lungs and spleens from the HKP1 orthotopic mouse lung adenocarcinoma model

Project description:To investigate how gene expression profiles change in CD8+ T cells based on time, tumor burden, and tissue localization, we orthotopically implanted the mouse lung adenocarcinoma HKP1 cell line expressing luciferase into syngeneic C57BL/6 mice by tail vein injections. Tumor growth was monitored by bioluminescence imaging. A separate cohort of mice received no tumor implantation. At days 7, 17, and 24 post tumor implantation, mice were euthanized and CD8+ T cells sorted from tumor-bearing lungs and spleens, or matched naive lungs and spleens. We then performed gene expression profiling analysis using data obtained from RNA-seq of sorted cells, comparing across time, tumor burden, and tissue localization.

2023-10-19 | GSE218141 | GEO

EZH2-dependent chromatin accessibility changes during B cell differentiation

Project description:To understand the role of EZH2 in B cell differentiation, EZH2 was inducibly deleted using tamoxifen and B cells stimulated to differentiate with LPS in vivo. After 3 days, EZH2-sufficient and EZH2-deficient naive B cells and plasmablasts were FACS isolated from the spleens and ATAC-seq was performed to identify the chromatin accessibility changes that are programmed by EZH2.

2017-12-16 | GSE103142 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data