Genomics

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MPE-seq, a New Method for the Genome-wide Analysis of Chromatin Structure


ABSTRACT: In this study we developed MPE-seq, a method for the genome-wide characterization of chromatin that involves the digestion of nuclei with methidiumpropyl-EDTA-Fe(II) [MPE-Fe(II)] followed by massively parallel sequencing. Like micrococcal nuclease (MNase), MPE-Fe(II) preferentially cleaves the linker DNA between nucleosomes. We also performed MNase-seq as a comparison. We further performed ChIP-seq using chromatin samples obtained by MPE-Fe(II) or MNase digestion of nuclei.

ORGANISM(S): Mus musculus

PROVIDER: GSE69098 | GEO | 2015/06/16

SECONDARY ACCESSION(S): PRJNA284528

REPOSITORIES: GEO

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