Project description:Normal young adult Sprague Dawley rats (male) 6 diaphragm muscle samples are compared with 6 sternohyoid muscle samples -- each sample is one muscle from one rat
Project description:Male Sprague Dawley adult rats were subjected to a cervical hemisection at C2 (C2HS). Costal diaphragm (both ipsilesional and contralesional sides) were assessed under control conditions (sham surgery) and at 1 and 7 days post-C2HS. We used SA Biosciences Rat Skeletal Muscle Development and Disease RT2 Profiler PCR Array to quantitate gene expression of muscle atrophy and regeneration-relevant genes from the uninjured and injured tissues on the indicated sides and at the indicated timepoints post-lesion.
Project description:Male Sprague-Dawley rats were randomly divided into (1) mock surgery (MS) group, mock surgery after EP (EPMS) group, myocardial ischemia (MI) group and MI after EP (EPMI) group. Twenty-four hours after MI or mock surgery , left ventricular tissues below the ligation were collected for the iTRAQ proteomics.
Project description:Proteomic analysis of diaphragm tissue from rats subjected to chronic heart failure. Redox sensitive Cysteine residues from both adult and old diaphragm tissues examined
Project description:Proteomic analysis of young and old murine diaphragm and associated changed in contractile aproperties. Redox sensitive Cysteine residues from both adult and old diaphragm tissues examined.
Project description:8 week old rats injected with streptozotocin or buffer alone at age of 8 weeks, heart obtained at 12 weeks (thus animals were diabetic for 4 weeks). Left vent of heart. Keywords: Disease state analysis (diabetes)
Project description:Male Sprague Dawley adult rats were subjected to a cervical hemisection at C2 (C2HS). Costal diaphragm (both ipsilesional and contralesional sides) were assessed under control conditions (sham surgery) and at 1 and 7 days post-C2HS. We used SA Biosciences Rat Skeletal Muscle Development and Disease RT2 Profiler PCR Array to quantitate gene expression of muscle atrophy and regeneration-relevant genes from the uninjured and injured tissues on the indicated sides and at the indicated timepoints post-lesion. qPCR gene expression profiling. Tissue was derived from the costal diaphragm from control animals, and from contralesional and ipsilesional sides at days 1 and 7 post-lesion. RNA was extracted from flash-frozen tissue (TRIzol reagent) and quantitated via spectrophotometry. Equal amount total RNA from each donor was pooled prior to gene expression analysis. N=3 or 4 rats per condition. Four genes in the array study appeared to undergo unphysiologic increases in expression following SCI: Mmp9, Leptin, activin A and alpha actinin. For these genes, expression levels in control tissue were detectable but extremely low, thus fold-increases following SCI are numerically exaggerated. Therefore, we cannot explicitly comment on the physiologic scale of this response