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Expression data for hindlimb and tail samples in a metamorphosis assay for thyroid axis disruption - inhibitors

ABSTRACT: Thyroid hormones (TH), thyroxine (T4) and 3, 5, 3’-triiodothyronine (T3), play crucial roles in regulation of growth, development and metabolism in vertebrates and are targets for endocrine disruptive agents. Perturbations in TH action can contribute to the development of disease states and the U.S. Environmental Protection Agency is developing a high throughput screen using TH-dependent metamorphosis of the Xenopus laevis tadpole as an assay platform. Currently this methodology relies on external morphological endpoints and changes in central thyroid axis parameters. However, exposure-related changes in gene expression in TH-sensitive tissue types that occur over shorter time frames have the potential to augment this screen. Using a combination of cDNA array and real time quantitative polymerase chain reaction (QPCR) analyses, this study identifies molecular markers in tissues peripheral to the central thyroid axis. We examine the hindlimb and tail of tadpoles up to 96 hours of continuous exposure to T3, T4, methimazole, propylthiouracil, or perchlorate. Several novel biomarker candidates are indicated that include transcripts encoding importin, RNA helicase II/Gu, and defender against death protein, DAD1. In combination with previously-identified biomarker candidates, these transcripts will greatly augment the predictive and diagnostic power of the Xenopus metamorphosis assay for perturbation of TH action. Keywords: time course

ORGANISM(S): Xenopus laevis Aquarana catesbeiana

PROVIDER: GSE7051 | GEO | 2007/05/12

SECONDARY ACCESSION(S): PRJNA98515

REPOSITORIES: GEO

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Expression data for brain samples in a metamorphosis assay for thyroid axis disruption - inhibitors

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Expression data from stage 54 Xenopus laevis larvae treated with 20 nM triiodothyronine (T3)

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