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Thyroid hormone induced changes in Xenopus laevis tadpole limb bud

ABSTRACT: Premetamorphic Xenopus laevis tadpoles limb bud cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in developing tadpole limb bud and compare it to TH-induced proliferation and differentiation program in tadpole brain. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. They were then treated with 100 nM T3 in 1 mM methimazole and 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). Limb buds were dissected at the end of the experiment. Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design

OTHER RELATED OMICS DATASETS IN: PRJNA93535PRJNA93539PRJNA93541

ORGANISM(S): Xenopus laevis unidentified

PROVIDER: GSE3404 | GEO | 2005/10/04

SECONDARY ACCESSION(S): PRJNA93539

REPOSITORIES: GEO

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Thyroid hormone induced changes in Xenopus laevis tadpole brain

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