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Dramatic Increase in Cell-subset Specific Methylation Accompanies Mammalian Brain Development [hairpin BS-Seq]

ABSTRACT: Purpose: This study aims to identify cell-subset specific methylated (CSM) loci in mammalian brain methylomes and to uncover epigenetic regulatory networks associated with brain development. Methods: To generate methylation data from DNA double strands simultaneously, genome-scale hairpin bisulfite sequencing technique was used for the assessment of the levels of asymmetric DNA methylation in the human frontal cortical gray and white matters Results and conclusion: CSM loci increase dramatically during early stages of brain development. They are overrepresented in cis-regulatory elements such as active enhancers and promoters, and are enriched for GWAS variants associated with neurological-disorder-related diseases/traits. Co-methylated CSM clustering analysis indicated that the CSMs hypo-methylated in neurons are enriched for EGR1 binding sites.Our results show the functional relevance of CSM loci identified and provide insights into epigenetic complexity of mammalian brains.

ORGANISM(S): Homo sapiens

PROVIDER: GSE70982 | GEO | 2017/02/14

SECONDARY ACCESSION(S): PRJNA290077

REPOSITORIES: GEO

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