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RNA-seq following enhancer repression

ABSTRACT: Using RNA-seq to identify genes regulated by dCas9-KRAB mediated enhancer repression in NCI-H2009 lung adenocarcinoma cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE72001 | GEO | 2015/10/26

SECONDARY ACCESSION(S): PRJNA292701

REPOSITORIES: GEO

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RNA-seq following enhancer repression

Project description:Using RNA-seq to identify genes regulated by dCas9-KRAB mediated enhancer repression in NCI-H2009 lung adenocarcinoma cells NCI-H2009 cells were first transfected with dCas9-KRAB fusion and subsequently transfected with no guide RNA (Empty), a guide RNA that has no target in the human genome (Dummy), and two separate guide RNAs (e3 #1 and e3 #2) recognizing the enhancer region of our study.

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