Transcriptomics

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The immunomodulatory effects of soluble receptor of activated nuclear factor kappa ligand (sRANKL) on monocyte-derived macrophages


ABSTRACT: To explore the effects of sRANKL on tumor-associated macrophages, we first sorted CD14+ monocytes from peripheral blood mononuclear cells of healthy donors using magnetic-activated cell sorting and then induced their differentiation into macrophages by culture with M-CSF for 5 days. We then stimulated these macrophages with IL-4 (20 ng/ml) together with or without 100 ng/ml of sRANKL for 6 hours and examined gene expression using a cDNA microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE72292 | GEO | 2015/08/24

SECONDARY ACCESSION(S): PRJNA293673

REPOSITORIES: GEO

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