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Refining mouse annotations via targeted RNA sequencing

ABSTRACT: In this work we aim to improve the understanding of the mouse transcriptome complexity, investigate the expressed fraction of the genome and ameliorate the available mouse annotations. We utilized CatureSeq, a recently described strategy meant to enhance the sequencing coverage of low abundant genes. In our experimental design we generated oligonucleotide probes to select annotated and putative long-noncoding RNA and splice junctions. This allowed us to improve dramatically the sequencing throughput of the targeted regions. As a consequence our approach permitted the simultaneous identification of thousands of exons and the expansion of the already known ones

ORGANISM(S): Mus musculus

PROVIDER: GSE72311 | GEO | 2016/04/04

SECONDARY ACCESSION(S): PRJNA293710

REPOSITORIES: GEO

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Refining mouse annotations via targeted RNA sequencing

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