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Dicer conditional knockout embryonic heart mutant vs control array

ABSTRACT: The goal is to find out the gene expression changes when Dicer was inactivated in the murine heart. Keywords: Dicer mutant

ORGANISM(S): Mus musculus

PROVIDER: GSE7343 | GEO | 2007/03/24

SECONDARY ACCESSION(S): PRJNA100449

REPOSITORIES: GEO

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Dicer conditional knockout embryonic heart mutant vs control array

Project description:The goal is to find out the gene expression changes when Dicer was inactivated in the murine heart. Keywords: Dicer mutant Dicer gene was specifically inactivated in the mouse heart using cardiac specific Nkx2.5 Cre knock-in line. Embryonic day 11.5 heart from mutant and control hearts were compared in triplicates.

2010-06-11 | E-GEOD-7343 | biostudies-arrayexpress

RNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which gene pathways were affected by Dicer loss, RNA sequencing was performed in whole neural retinas of P21 control and Dicer CKO mice. This experiment aims to identify which genes were differentially expressed between Dicer conditional knockout and control mice.

2019-01-14 | E-MTAB-6133 | biostudies-arrayexpress

miRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which miRNAs were involved in such phenotype, miRNA sequencing was performed in whole retinas of P21 and 3.5 month old control and Dicer CKO mice. This experiment aims to identify which miRNAs were differentially expressed in Dicer conditional knockout mice when compared to control mice.

2019-01-14 | E-MTAB-6132 | biostudies-arrayexpress

Phosphoproteomic analysis of liver of Dicer knockout mice

Project description:Dicer is an essential ribonuclease involved in the biogenesis of miRNA. Previous studies have reported that Dicer is dysregulated in multiple types of cancers. To investigate the downstream phosphoproteome of Dicer, we carried out phosphotyrosine profiling of the liver of Dicer knockout of mice. We employed antibody-based enrichment of phosphotyrosine containing peptides coupled with spike-in SILAC-based quantitation. Over 400 phosphoisites were identified in each condition while 290 phosphosites were quantitated in common. Amongst these, several key signaling molecules like receptor tyrosine kinase MET, MET, PDGFR, EPHA2, EPHB4 and IGF1R/INSR were hyperphosphorylated. Also, non-receptor tyrosine kinases of Src family and their downstream effector signaling partners including IRS2 and STAT3were found to be hyperphosphorylated. Our study indicates that there is significant alteration in the signaling pathways upon ablation of Dicer.

2016-07-08 | PXD000927 | Pride

miRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:miRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

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RNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

Project description:RNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas

| PRJEB30776 | ENA

Transcriptional profiling of Dicer conditional knockout hematopoietic stem cells.

Project description:Gene expression of HSC (Lin-Sca-Kit+Flt3-) hematopoietic stem cells from Dicer D/+ mice was compared with LT-HSC from DicerD/D mice. BM cells were noncompetitively transplanted into irradiant recipients, and deletion induced by poly(IC) injection. Donor-derived HSCs were isolated by FACS sorting of recipient bone marrow. Biological replicates of each (2 per genotype) were generated and expression profiles were determined by hybridization to Affymetrix Moe430_2 arrays.

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Dicer knockout mouse subcutaneous preadipocytes

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Dicer knockout NSCLC RNAseq and miRseq

Project description:Dicer knockout NSCLC mRNAseq profiles the transcriptome, Dicer knockout NSCLC miRseq profiles the miRnome

2014-04-25 | GSE57043 | GEO

Dicer knockout mouse subcutaneous preadipocytes

Project description:Dicer is required for miRNA processing and RNA interference. Here we knocked out Dicer in SV40 T antigen immortalized subcutaneous preadipocytes isolated from Dicer flox mice by transducing these cells with adenoviruses harboring Cre recombinase. Control cells were transduced with adenovirus harboring GFP. We used microarrays to detail the gene expression profile of Dicer knockout preadipocytes. Four days after adenovirus infection, cells were harvested for RNA isolation and hybridization on Affymetrix microarrays. At this point, Dicer knockout was confirmed by several methods.

2012-03-30 | E-GEOD-24683 | biostudies-arrayexpress

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