Dicer-2 senses a 5' secondary structural element of picorna-like viruses for priming antiviral RNA interference
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ABSTRACT: Introduction: Insects as well as plants base their innate antiviral immunity on RNA interference (RNAi). In the model organism Drosophila melanogaster the replicating virus is sensed by the RNase III enzyme Dicer-2, which processes double stranded (ds) viral RNA into 21 nt long small interfering (si) RNAs. A distinctive advantage of the fly antiviral immune system is that these siRNAs provide a footprint of its action. Methods: We use a combination of high throughput sequencing and genetics to unravel the mechanisms involved in viral RNA sensing in Drosophila. Particularly, we cloned and sequenced small RNAs from virus-infected flies. The sequencing was performed by Illumina HiSeq2000. Sequence strategy was 1 x 50 base pairs (bp). Results: We identify a conserved RNA structure in the virus that serves as entry point for Dicer-2 to initiate RNA interference. The recognition of this structure is independent to the helicase activity of the protein. However, the hydrolysis of ATP is essential to mantain the enzyme fully processive.
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE76101 | GEO | 2024/12/20
SECONDARY ACCESSION(S): PRJNA306225
REPOSITORIES: GEO
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