Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data of human tympanic membrane cells and epidermal keratinocytes

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE76674 | GEO | 2016/01/08

SECONDARY ACCESSION(S): PRJNA308303

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data of human tympanic membrane cells and epidermal keratinocytes

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-01-08 | E-GEOD-76674 | biostudies-arrayexpress

Expression data of human tympanic membrane cells and epidermal keratinocytes

Project description:The data set compares the gene expression of two types of keratinocytes from the tympanic membrane and epidermis of the body. Healthy human samples were harvested for the analysis. Cells were cultivated to evaluate the effekt of keratinocytes only. 626 Chip Ids considered differentially expressed between the cell types. Enrichment tests show genes related to migration are over-represented in the highly expressed genes of TMK vs. EK. Six samples were analysed in total. Each cell type in biological triplicates. Genes of p-value <0.05 and fold change of ≥ 1.5 were considered significant.

2014-11-04 | E-GEOD-62939 | biostudies-arrayexpress

Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]

2016-05-31 | GSE76673 | GEO

Expression data of human tympanic membrane cells and epidermal keratinocytes [mRNA]

2014-11-04 | GSE62939 | GEO

Transcription and microRNA Profiling of Cultured Human Tympanic Membrane Epidermal Keratinocytes.

Project description:The human tympanic membrane (TM) has a thin outer epidermal layer which plays an important role in TM homeostasis and ear health. The specialised cells of the TM epidermis have a different physiology compared to normal skin epidermal keratinocytes, displaying a dynamic and constitutive migration that maintains a clear TM surface and assists in regeneration. Here, we characterise and compare molecular phenotypes in keratinocyte cultures from TM and normal skin. TM keratinocytes were isolated by enzymatic digestion and cultured in vitro. We compared global mRNA and microRNA expression of the cultured cells with that of human epidermal keratinocyte cultures. Genes with either relatively higher or lower expression were analysed further using the biostatistical tools g:Profiler and Ingenuity Pathway Analysis. Approximately 500 genes were found differentially expressed. Gene ontology enrichment and Ingenuity analyses identified cellular migration and closely related biological processes to be the most significant functions of the genes highly expressed in the TM keratinocytes. The genes of low expression showed a marked difference in homeobox (HOX) genes of clusters A and C, giving the TM keratinocytes a strikingly low HOX gene expression profile. An in vitro scratch wound assay showed a more individualised cell movement in cells from the tympanic membrane than normal epidermal keratinocytes. We identified 10 microRNAs with differential expression, several of which can also be linked to regulation of cell migration and expression of HOX genes. Our data provides clues to understanding the specific physiological properties of TM keratinocytes, including candidate genes for constitutive migration, and may thus help focus further research.

| S-EPMC5962475 | biostudies-literature

Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]

Project description:The data set compares the microRNA expression of two types of keratinocytes from the tympanic membrane and epidermis of the body. Healthy human samples were harvested for the analysis. Cells were cultivated to evaluate the effect of keratinocytes only. Few Chip Ids (11) were considered differentially expressed between the cell types. Six samples were analyzed in total. Each cell type in biological triplicates. Genes of p-value <0.05 and fold change of â¥ 1.5 were considered significant.

2016-05-31 | E-GEOD-76673 | biostudies-arrayexpress

Tenascin-C expressing touch dome keratinocytes exhibit characteristics of all epidermal lineages.

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-29 | GSE228989 | GEO

Gata6 network regulates epidermal junctional zone cell fate.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2017-05-16 | E-GEOD-62608 | biostudies-arrayexpress

Genome-wide mRNA and miRNA expression data from different populations of human thymic epithelial cells (hTECs) compared to human epidermal derived keratinocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-07-31 | GSE85481 | GEO

ChIP-seq and RNA-seq of Human progenitor epidermal keratinocytes (HPEK) and HaCaT cells

Project description:This SuperSeries is composed of the SubSeries listed below.