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Expression data from Jurkat Clone E-6 cells exposed to nanosecond duration electrical pulses

ABSTRACT: It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

ORGANISM(S): Homo sapiens

PROVIDER: GSE77907 | GEO | 2016/02/16

SECONDARY ACCESSION(S): PRJNA311997

REPOSITORIES: GEO

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Expression data from Jurkat Clone E-6 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress Jurkat cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77907 | biostudies-arrayexpress

Expression data from HDFA cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress HDFA cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77906 | biostudies-arrayexpress

Expression data from HDFA cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

2016-02-16 | GSE77906 | GEO

Expression data from U-937 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress U-937 cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77908 | biostudies-arrayexpress

Expression data from U-937 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress

2016-02-16 | GSE77908 | GEO

Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP).

Project description:Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.

| S-EPMC4852903 | biostudies-literature

Adult human dermal fibroblasts exposed to nanosecond electrical pulses exhibit genetic biomarkers of mechanical stress.

Project description:BackgroundExposure of cells to very short (<1 µs) electric pulses in the megavolt/meter range have been shown to cause a multitude of effects, both physical and molecular in nature. Physically, nanosecond electrical pulses (nsEP) can cause disruption of the plasma membrane, cellular swelling, shrinking and blebbing. Molecularly, nsEP have been shown to activate signaling pathways, produce oxidative stress, stimulate hormone secretion and induce both apoptotic and necrotic death. We hypothesize that studying the genetic response of primary human dermal fibroblasts exposed to nsEP, will gain insight into the molecular mechanism(s) either activated directly by nsEP, or indirectly through electrophysiology interactions.MethodsMicroarray analysis in conjunction with quantitative real time polymerase chain reaction (qRT-PCR) was used to screen and validate genes selectively upregulated in response to nsEP exposure.ResultsExpression profiles of 486 genes were found to be significantly changed by nsEP exposure. 50% of the top 20 responding genes coded for proteins located in two distinct cellular locations, the plasma membrane and the nucleus. Further analysis of five of the top 20 upregulated genes indicated that the HDFa cells' response to nsEP exposure included many elements of a mechanical stress response.ConclusionsWe found that several genes, some of which are mechanosensitive, were selectively upregulated due to nsEP exposure. This genetic response appears to be a primary response to the stimuli and not a secondary response to cellular swelling.General significanceThis work provides strong evidence that cells exposed to nsEP interpret the insult as a mechanical stress.

| S-EPMC5614618 | biostudies-literature

Gene expression induced by Nanosecond pulsed electric fields

Project description:We use a human whole genome microarray to analyze the effects of nanosecond pulsed electric fields on Jurkat cells with the focus on early response genes to DNA damage. Keywords: nanosecond pulsed electric fields, jurkat cells, DNA damage

2006-02-01 | GSE4106 | GEO

Homo sapiens

Project description:Expression data from HDFA cells exposed to nanosecond duration electrical pulses

| PRJNA311994 | ENA

Cellular Processes Involved in Jurkat Cells Exposed to Nanosecond Pulsed Electric Field.

Project description:Recently, nanosecond pulsed electric field (nsPEF) has been considered as a new tool for tumor therapy, but its molecular mechanism of function remains to be fully elucidated. Here, we explored the cellular processes of Jurkat cells exposed to nanosecond pulsed electric field. Differentially expressed genes (DEGs) were acquired from the GEO2R, followed by analysis with a series of bioinformatics tools. Subsequently, 3D protein models of hub genes were modeled by Modeller 9.21 and Rosetta 3.9. Then, a 100 ns molecular dynamics simulation for each hub protein was performed with GROMACS 2018.2. Finally, three kinds of nsPEF voltages (0.01, 0.05, and 0.5 mV/mm) were used to simulate the molecular dynamics of hub proteins for 100 ns. A total of 1769 DEGs and eight hub genes were obtained. Molecular dynamic analysis, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), and the Rg, demonstrated that the 3D structure of hub proteins was built, and the structural characteristics of hub proteins under different nsPEFs were acquired. In conclusion, we explored the effect of nsPEF on Jurkat cell signaling pathway from the perspective of molecular informatics, which will be helpful in understanding the complex effects of nsPEF on acute T-cell leukemia Jurkat cells.

| S-EPMC6929111 | biostudies-literature

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