Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress HDFA cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure
Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress U-937 cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure
Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress Jurkat cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure
Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress
Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress
Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress
Project description:We show that exposure of artificial human skin tissue to intense, picosecond-duration THz pulses affects expression levels of numerous genes associated with non-melanoma skin cancers, psoriasis and atopic dermatitis. Genes affected by intense THz pulses include nearly half of the epidermal differentiation complex (EDC) members. EDC genes, which are mapped to the chromosomal human region 1q21, encode for proteins that partake in epidermal differentiation and are often overexpressed in conditions such as psoriasis and skin cancer. In nearly all the genes differentially expressed by exposure to intense THz pulses, the induced changes in transcription levels are opposite to disease-related changes. Total RNA from exposed artificial human skin tissues to picosecond-duration broadband (0.2–2.5 THz) THz pulses with 1 kHz repetition rate, 1/e2 spot-size diameter of 1.5 mm and pulse energies of either 1.0 ?J or 0.1 ?J. For comparison, we have exposed skin tissues to UVA pulses (400 nm, 0.1 ps, 0.024 ?J).
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
