Transcriptomics

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Gene expression profiling of s-SHIP positive mammary epithelial cells


ABSTRACT: We performed RNAseq on subpopulations of mammary epithelial cells. We carried out sorting of a gradient of s-SHIP positive cells in the mammary gland (neg, low, and hi for s-SHIP eGFP). High sSHIP-eGFP populations denote a postulated stem cell population, while low and negative represent more differentiated cell types. s-SHIP eGFP hi to negative potentially represents a gradient from stem to more differentiated progeny, respectively, within the basal epithelial compartment. We FACS sorted 3 replicates for each cell type to represent s-SHIP-neg, s-SHIP-low, and s-SHIP-high.

ORGANISM(S): Mus musculus

PROVIDER: GSE81385 | GEO | 2017/11/07

SECONDARY ACCESSION(S): PRJNA321426

REPOSITORIES: GEO

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