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Gene expression analysis of T cell subsets infiltrating lacrimal glands from the NOD mouse model of Sjögren's

ABSTRACT: Despite being one of the most common rheumatologic diseases, there is still no disease-modifying drug for primary Sjögren’s syndrome (pSS). Advancing our knowledge of the target tissue has been limited by the low dimensionality of histology techniques and the small size of human salivary gland biopsies. The goal of this study was to characterize CD4+ T cell subsets infiltrating the target tissue of the disease. We took advantage of a molecularly-validated mouse model of pSS (the NOD mouse model) and used cytometry techniques (flow and mass cytometry) in combination with transcriptional analyses. We identified three novel CD4+ T cell subsets that were not previously described in pSS pathogenesis and this GEO dataset reports the transcriptomic analysis of each of these three subsets: (1) effector Tregs (eTregs) expressing high levels of PD1 and ICOS as well as many immunoregulatory factors such as Il10, Areg, Fgl2, Itgb8; (2) Il21+ Th1 cells that also expressed high levels of PD1 and ICOS as well as osteopontin and shared striking transcriptional similarities with a pathogenic T follicular subset described in several lupus mouse models; (3) naïve PD1neg ICOSneg CD4+ T cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE81621 | GEO | 2016/09/01

SECONDARY ACCESSION(S): PRJNA322134

REPOSITORIES: GEO

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