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Gene expression analysis of total B, CD4+ T and epithelial cells in lacrimal glands from the NOD mouse model of Sjogren's syndrome

ABSTRACT: Despite being one of the most common rheumatologic diseases, there is still no disease-modifying drug for primary Sjögren’s syndrome (pSS). Advancing our knowledge of the target tissue has been limited by the low dimensionality of histology techniques and the small size of human salivary gland biopsies. The goal of this study was to characterize the gene expression profile of total B cells, CD4+ T cells and epithelial cells in the target tissue of the most commonly-used model of pSS (the NOD model of pSS characterized by severe dacryoadenitis developing in males between 8 and 16 weeks of age).

ORGANISM(S): Mus musculus

PROVIDER: GSE83113 | GEO | 2016/09/01

SECONDARY ACCESSION(S): PRJNA324806

REPOSITORIES: GEO

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Gene expression analysis of T cell subsets infiltrating lacrimal glands from the NOD mouse model of Sjögren's

Project description:Despite being one of the most common rheumatologic diseases, there is still no disease-modifying drug for primary Sjögren’s syndrome (pSS). Advancing our knowledge of the target tissue has been limited by the low dimensionality of histology techniques and the small size of human salivary gland biopsies. The goal of this study was to characterize CD4+ T cell subsets infiltrating the target tissue of the disease. We took advantage of a molecularly-validated mouse model of pSS (the NOD mouse model) and used cytometry techniques (flow and mass cytometry) in combination with transcriptional analyses. We identified three novel CD4+ T cell subsets that were not previously described in pSS pathogenesis and this GEO dataset reports the transcriptomic analysis of each of these three subsets: (1) effector Tregs (eTregs) expressing high levels of PD1 and ICOS as well as many immunoregulatory factors such as Il10, Areg, Fgl2, Itgb8; (2) Il21+ Th1 cells that also expressed high levels of PD1 and ICOS as well as osteopontin and shared striking transcriptional similarities with a pathogenic T follicular subset described in several lupus mouse models; (3) naïve PD1neg ICOSneg CD4+ T cells.

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Alterations in gene expression in lacrimal and salivary glands of male NOD mice due to LTBR-Ig treatment relative to control antibody

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2018-03-21 | PXD008199 | Pride

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Proteomics of saliva, plasma, and salivary gland tissue in Sjögren’s syndrome and non-Sjögren patients identify novel biomarker candidates

Project description:Objective: The aim of this study was to characterize and compare the proteome in whole saliva, plasma, and salivary gland tissue in patients with primary Sjögren’s syndrome (pSS) and patients having symptoms of pSS, but not fulfilling the classification criteria, and to search for diagnostic biomarker candidates for pSS. Methods: Liquid chromatography tandem mass spectrometry was conducted on whole saliva, plasma, and labial salivary gland tissue samples from 24 patients with pSS and 16 non-Sjögren control subjects (non-pSS). Gene Ontology (GO)-terms and Kyoto Encyclopedia of Genes and Genomes (KEGG)-pathways were applied for functional annotation. Results: 1,013 proteins were identified in whole saliva, 219 in plasma, and 3,166 in salivary gland tissue. In saliva, 40 proteins differed significantly between the two groups. In pSS, proteins involved in immunoinflammatory processes were upregulated, whereas proteins related to salivary secretion were downregulated. The combination of neutrophil elastase, calreticulin, and tripartite motif-containing protein 29 yielded a receiver-operating characteristic (ROC) value of 0.97 (CI 0.93-1.00). Protein expression in plasma and salivary gland tissue did not differ between the patient groups. Conclusion: The salivary proteome of patients with pSS differed from that of non-pSS patients, indicating that saliva proteomics represents a promising non-invasive diagnostic tool for pSS.

2020-07-09 | PXD016231 | Pride

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