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Identification of DHT induced cell cycle dependent trascriptome and AR binding events

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE82203 | GEO | 2016/10/05

SECONDARY ACCESSION(S): PRJNA324290

REPOSITORIES: GEO

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Identification of DHT induced cell cycle dependent trascriptome and AR binding events

Project description:Identification of DHT induced cell cycle dependent trascriptome and AR binding events

| PRJNA324290 | ENA

Identification of cell cycle regulated, DHT induced AR binding events

Project description:The androgen receptor (AR) is required throughout all stages of disease progression. Despite this, little is known about the function of AR in the mitotic cell cycle. Here, we report novel AR binding as a result of examining the AR cistrome in actively cycling cells.

2016-10-05 | GSE82202 | GEO

Identification of DHT induced cell cycle dependent trascriptome in prostate cancer cells

Project description:Evaluation of the genome wide impact of cell cycle position on DHT stimulated gene expression programs. Results show differential cell cycle regulated gene expression in different cell cycle phases.

2016-10-05 | GSE82201 | GEO

DHT-AR, E2-AR and R5020-AR target gene profiles in LNCaP

Project description:We previously encountered regulatory processes where dihydrotestosterone (DHT) exerted its inhibitory effect on parathyroid hormone-related protein (PTHrP) gene repression through the estrogen receptor (ER)α, but not the androgen receptor (AR) in breast cancer MCF-7 cells. Here, we investigated whether such an aberrant ligand-nuclear receptor (NR) interaction is present in prostate cancer LNCaP cells. First, we confirmed that LNCaP cells expressed a functional AR and at negligible levels of ERα, and progesterone receptors. Both suppression of PTHrP and activation of the PSA genes were observed after treatment of E2, DHT and R5020. Consistent with the previous notion that the AR in LNCaP cells lost the ligand specificity due to a mutation AR (Thr-Ala877), our study using siRNA targeting each NR revealed that the AR, but not the other NRs, monopolized the role as the mediator of shared hormone-dependent regulation. These results were invariably associated with nuclear translocation of this mutant AR. Microarray of the genes regulated by either DHT, E2 or R5020 downstream of the AR (Thr-Ala877) revealed that more than half genes overlapped in LNCaP cells. Noticeably, AR (wild-type, wt) and AR (Thr-Ala877) were equally responsible for the E2-AR interactions. Fluorescent microscopic experiments demonstrated that both EGFP-AR (wt) and EGFP-AR (Thr-Ala877) were exclusively localized within the nucleus after E2 or DHT treatment. Further, a promoter assay revealed that breast cancer MCF-7 and Rv22 cells also exhibited such an aberrant E2-AR (wt) signaling. We postulate entangled interactions between the AR (wt) and E2 in a certain hormone-sensitive cancer cells. Total RNAs from the LNCaP cells transfected with control siRNA (siCT) or siRNA for AR (siAR) transfected LNCaP cells before 24 hr followed by exposed to 10-7M of DHT, E2 or R5020 exposure for another 24 h, respectively, were used.

| S-ECPF-GEOD-58615 | biostudies-other

DHT-dependent AR activity in LNCaP cells

Project description:AR transcriptional activity is regulated by DHT

2016-03-15 | GSE60721 | GEO

Mapping of DHT-responsive or -independent AR-binding sites induced by activated Src in prostate cancer cell lines

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-12-20 | GSE92576 | GEO

The AR dependent cell cycle: mechanisms and cancer relevance.

Project description:Prostate cancer cells are exquisitely dependent on androgen receptor (AR) activity for proliferation and survival. As these functions are critical targets of therapeutic intervention for human disease, it is imperative to delineate the mechanisms by which AR engages the cell cycle engine. More than a decade of research has revealed that elegant intercommunication between AR and the cell cycle machinery governs receptor-dependent cellular proliferation, and that perturbations in this process occur frequently in human disease. Here, AR-cell cycle interplay and associated cancer relevance will be reviewed.

| S-EPMC3641823 | biostudies-literature

The identification of RBM47 binding sites and RBM47-dependent alternative splicing events in brain metastatic breast cancer cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-11 | E-GEOD-58381 | biostudies-arrayexpress

Finasteride accelerates prostate wound healing after thulium laser resection through DHT and AR signalling.

Project description:ObjectivesUrinary tract infection, urinary frequency, urgency, urodynia and haemorrhage are common post-operative complications of thulium laser resection of the prostate (TmLRP). Our study mainly focuses on the role of finasteride in prostate wound healing through AR signalling.Materials and methodsTmLRP beagles were randomly distributed into different treatment groups. Serum and intra-prostatic testosterone and DHT level were determined. Histological analysis was conducted to study the re-epithelialization and inflammatory response of the prostatic urethra in each group. We investigated the role of androgen in proliferation and inflammatory response in prostate. In addition, the effects of TNF-α on prostate epithelium and stromal cells were also investigated.ResultsTestosterone and DHT level increased in testosterone group and DHT decreased in finasteride group. Accelerated wound healing of prostatic urethra was observed in the finasteride group. DHT suppressed proliferation of prostate epithelium and enhanced inflammatory response in prostate. We confirmed that DHT enhanced macrophages TNF-α secretion through AR signalling. TNF-α suppressed proliferation of prostate epithelial cells and retarded cell migration. TNF-α also played a pivotal role in suppressing fibroblasts activation and contraction.ConclusionTestosterone treatment repressed re-epithelialization and wound healing of prostatic urethra. Finasteride treatment may be an effective way to promote prostate re-epithelialization.

| S-EPMC6528864 | biostudies-literature

Analyses of AR stimulated by 0.1 nM DHT in LNCaP stable cell line overexpressing AR

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-11-21 | GSE211641 | GEO