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Stb3 deletion affects gene expression within 10 minutes of glucose addition

ABSTRACT: Microarrays were conducted to asses the effect of Stb3 deletion in immediate transcriptional induction in response to glucose Keywords: Stb3, immediate glucose induction, growth genes.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE8379 | GEO | 2007/07/06

SECONDARY ACCESSION(S): PRJNA101417

REPOSITORIES: GEO

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Transcription profiling of Saccharomyces cerevisiae reveals Stb3 deletion affects gene expression within 10 minutes of glucose addition

Project description:Microarrays were conducted to asses the effect of Stb3 deletion in immediate transcriptional induction in response to glucose Experiment Overall Design: Response of Stb3 delete cells was compared to that of wild type (BY4742) in cells grown for 3 days in YPD and cells that received 2% glucose for 10 minutes after the 3 day growth period. Two repetitive samples were done for each timepoint.

2009-04-30 | E-GEOD-8379 | biostudies-arrayexpress

transcriptional reponse of yeast glucose sensing pathway mutants to glucose addition

Project description:While much is known about glucose metabolism in yeast, less is known about the receptors and signaling pathways that indicate glucose availability. Here we compared wildtype and 16 mutants in yeast glucose sensing pathway for their transcriptomics profiles in 0.05% glucose vs 10 min after glucose addition to 2% glucose. With these data, we were able to define various roles of glucose sensing pathway components. We demonstrated that the G protein-coupled receptor (Gpr1/Gpa2) directed early events in glucose utilization, the transceptors (SNF3/RGT2) regulated subsequent processes and downstream products of glucose metabolism. Whereas the large G protein transmits the signal from its cognate receptor, Ras2 (but not Ras1) integrates responses from both receptor pathways. We also determined the relative contributions of the Gα (Gpa2) and Gβ (Asc1) protein subunits to glucose-initiated processes in yeast. We determined that Gpa2 is primarily involved in regulating carbohydratesugar metabolism while Asc1 is primarily involved in amino acid metabolism. Both proteins are involved in regulating purine metabolism. Collectively, our analysis reveals the molecular basis for glucose detection and the earliest events of glucose-dependent signal transduction in yeast.

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transcriptional reponse of yeast glucose sensing pathway mutants to glucose addition

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Proteomic dataset on abnormal glucose tolerance versus healthy controls with 10 abnormal glucose tolerance. 10 in healthy controls

Project description:Proteomic dataset on abnormal glucose tolerance versus healthy controls with 10 abnormal glucose tolerance. 10 in healthy controls

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Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes.

Project description:BackgroundOxygen and glucose metabolism play pivotal roles in many (patho)physiological conditions. In particular, oxygen and glucose deprivation (OGD) during ischemia and stroke results in extensive tissue injury and cell death.ResultsUsing time-resolved ribosome profiling, we assess gene expression levels in a neural cell line, PC12, during the first hour of OGD. The most substantial alterations are seen to occur within the first 20 minutes of OGD. While transcription of only 100 genes is significantly altered during one hour of OGD, the translation response affects approximately 3,000 genes. This response involves reprogramming of initiation and elongation rates, as well as the stringency of start codon recognition. Genes involved in oxidative phosphorylation are most affected. Detailed analysis of ribosome profiles reveals salient alterations of ribosome densities on individual mRNAs. The mRNA-specific alterations include increased translation of upstream open reading frames, site-specific ribosome pauses, and production of alternative protein isoforms with amino-terminal extensions. Detailed analysis of ribosomal profiles also reveals six mRNAs with translated ORFs occurring downstream of annotated coding regions and two examples of dual coding mRNAs, where two protein products are translated from the same long segment of mRNA, but in two different frames.ConclusionsThese findings uncover novel regulatory mechanisms of translational response to OGD in mammalian cells that are different from the classical pathways such as hypoxia inducible factor (HIF) signaling, while also revealing sophisticated organization of protein coding information in certain genes.

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Compare the effects of sevoflurane or propofol on perioperative glucose metabolism in patients undergoing laparoscopic colorectal cancer surgery

Project description:Interventions: Group S:induction 2-3% and maintenance 1.5-2.5% of sevoflurane during operation;Group S+L:induction 2-3% and 1.5-2.5%maintenance of sevoflurane during operation, lipid emulsions 1mg/kg/h during operations;Group P:induction and maintenance of propofol 3.0-4.0ug/ml during operation Primary outcome(s): asprosin;blood glucose;insulin Study Design: Parallel

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Response of gcr1 null mutant to glucose

2006-11-27 | GSE5575 | GEO

B. thetaiotaomicron before and after glucose addition to tryptone media

Project description:B. thetaiotaomicron was grown in a batch culture fermenter containing tryptone media. Glucose (final concentration 0.2%) was added at OD600 = 0.3. Samples were taken immediately before and 60 minutes after addition. Keywords: time course

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Streptococcus mutans UA159; 10 mM Glucose Versus 100 mM Glucose

Project description:Transcriptional Profiling of Streptococcus mutans UA159 Grown in Continuous Culture using TV Media Supplemented With 10 mM vs 100 mM Glucose. The genetic and phenotypic responses of Streptococcus mutans, an organism known to be strongly associated with the development of dental caries, to changes in carbohydrate availability were investigated. S. mutans UA159 or a derivative of UA159 lacking ManL, which is the EIIAB component (EIIABMan) of a mannose/glucose permease of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) and a dominant effector of catabolite repression, were grown in continuous culture to steady-state in conditions of excess (100 mM) or limiting (10 mM) glucose. Microarrays using RNA from S. mutans UA159 revealed that 174 genes were differentially expressed in response to changes in carbohydrate availability (P < 0.001). Glucose-limited cells possessed higher PTS activity, could acidify the environment more rapidly and to a greater extent, and produced more ManL protein than cultures grown with excess glucose. Loss of ManL adversely affected carbohydrate transport and acid tolerance. Comp arison of the HPr protein in S. mutans UA159 and the manL deletion strain indicated that the differences in behaviors of the strains were not due to major differences in HPr pools or HPr phosphorylation status. Therefore, carbohydrate availability alone can dramatically influence the expression of physiologic and biochemical pathways that contribute directly to the virulence of S. mutans, and ManL has a profound influence on this behavior. Two-condition experiment, growth in 10 mM vs 100 mM glucose. Biological replicates: 3 per condition, independently grown and harvested. One replicate per array

2013-12-03 | E-GEOD-51362 | biostudies-arrayexpress

Response of gcr1 null mutant to glucose

Project description:This experiment was done to detect the transcriptomic defects in the gcr1 null mutant, relative to an isogenic wild type, in the immediate response to glucose. Keywords: time course Both gcr1 mutant and wild type cells were grown in YEP containing 3% pyruvate to early/mid log phase. The 0min sample was taken just prior to glucose addition. After addition of glucose, samples were taken at 20min and 60min. Total RNA was extracted for both strains and expression was measured by competitive hybridization of the mutant and wild type labeled cRNA for each time point.

2010-06-30 | E-GEOD-5575 | biostudies-arrayexpress

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