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Transcriptome analysis after knocking down class III HD ZIP transcription factors in SAM

ABSTRACT: We ubiqoutesly expressed mir165a (pUBQ>>mir165a) in ap1 cal background for RNA-Seq studies. Expression of this transgenes in ap1 cal background gives phenotype similar to their expression in wild-type (WT) backgrounds. We induced the transgenes for 8 hours or 16 hours. As a control we used pUBQ::GR-LHG4 transgeneic line. Q-PCR analysis on extracted RNA showed the successful down regulation of known target genes of class III HD ZIPs.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE90170 | GEO | 2020/04/16

REPOSITORIES: GEO

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