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Transcriptome analysis after ectopically expressing KAN1 in SAM epidermis

ABSTRACT: We ectopically expressed KAN1 in epidermis in ap1 cal background by using AtML1 promoter driven inducible KAN1-2GFP for RNA-Seq studies. Expression of this transgenes in ap1 cal background gives phenotype similar to their expression in wild-type (WT) backgrounds. For pML1>>KAN1-2GFP , we sorted the epidermal cells using FACS, after their induction of 6 hours or 16 hours. As a control we collected the epidermal cells after 6 or 16hr induction of pML1::GR-LHG4 in pML1::BFPer background. Q-PCR analysis on extracted RNA showed the successful sorting and regulation of known target genes of KAN1.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE90507 | GEO | 2020/04/16

REPOSITORIES: GEO

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