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Transcriptome analysis after ectopically expressing KAN1 in SAM epidermis

ABSTRACT: We ectopically expressed KAN1 in epidermis in ap1 cal background by using AtML1 promoter driven inducible KAN1-2GFP for RNA-Seq studies. Expression of this transgenes in ap1 cal background gives phenotype similar to their expression in wild-type (WT) backgrounds. For pML1>>KAN1-2GFP , we sorted the epidermal cells using FACS, after their induction of 6 hours or 16 hours. As a control we collected the epidermal cells after 6 or 16hr induction of pML1::GR-LHG4 in pML1::BFPer background. Q-PCR analysis on extracted RNA showed the successful sorting and regulation of known target genes of KAN1.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE90507 | GEO | 2020/04/16

REPOSITORIES: GEO

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Transcriptome analysis after ectopically expressing REV in SAM epidermis

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Ath_Agilent_v2_Riechmann array expression profile AP1-GR ap1cal inflorescences - chx experiment

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Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation

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2017-04-28 | GSE96799 | GEO

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Ath_Agilent_v2_Riechmann array expression profile AP1-GR ap1cal inflorescences - chx experiment

2011-01-01 | GSE20175 | GEO

Expression profile pAP1-AP1-GR ap1cal inflorescences - time series (days)

2014-01-01 | GSE47981 | GEO

Expression profile pAP1-AP1-GR ap1cal inflorescences - time series (days)

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2014-01-01 | E-GEOD-47981 | biostudies-arrayexpress

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