Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

ABSTRACT: The purpose of this experiment was to identify the genes bound by Ndt80 in P. pastoris when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

ORGANISM(S): Komagataella pastoris

PROVIDER: GSE90663 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA355300

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae when constitutively expressed, for the native Ndt80 as well as a heterologous Ndt80 from Pichia pastoris. For the ChIPs on the native Ndt80s, Ndt80 was tagged with c-myc; for the heterologous ChIP P. pastoris Ndt80 was integrated into the S. cerevisiae genome and tagged with c-myc. In both cases, the native Ndt80 promoter was also replaced with an A. gossypii Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90660 | GEO

ChIP-Seq of constitutively expressed Ndt80A-Myc and Ndt80B-Myc in S. stipitis

Project description:The purpose of this experiment was to identify the genes bound by the two Ndt80 paralogs in S. stipitis when constitutively expressed. Both paralogs were tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90664 | GEO

ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in K. lactis when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the K. lactis Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90662 | GEO

Global gene expression in P. pastoris during mitotic and meiotic growth, with and without meiotic regulator Ndt80

Project description:The purpose of this experiment was to identify the genes with increased expression during sporulation in wild-type P. pastoris, and during sporulation in cells with Ndt80 deleted. Cells were grown unti log-phase in YPD or in sporulation media (0.5% sodium acetate, 1% potassium chloride, 1% glucose, 2% agar plates ) for 30 hours. Each experiment was repeated three times and sequenced on an Illumina HiSeq 4000

2017-05-01 | GSE92766 | GEO

ChIP-Seq of constitutively expressed Ndt80A-Myc and constitutively and endogenously expressed Ndt80B-Myc in C. albicans

Project description:The purpose of this experiment was to identify the genes bound by the two Ndt80 paralogs in C. albicans when constitutively expressed, and for Ndt80B when endogenously expressed. Both paralogs were tagged with c-myc, and the protein was immunoprecipitated with a c-myc antibody. For constitutive expression, each native Ndt80 promoter was replaced by the C. albicans Tdh3 promoter. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90665 | GEO

Komagataella pastoris

Project description:ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

| PRJNA355300 | ENA

ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90661 | GEO

ChIP-seq of Ndt80 in a C. albicans x C. dubliniensis hybrid during biofilm formation

Project description:The purpose of this experiment was to identify the genes bound by the regulator Ndt80 in a C. albicans x C. dubliniensis hybrid during biofilm formation. C. albicans or C. dubliniensis Ndt80 was tagged with a c-myc epitope in a C. albicans x C. dubliniensis hybrid and the immunoprecipitation of the tagged strain was compared to that of an untagged control hybrid strain.

2021-04-14 | GSE161134 | GEO

ChIP-seq of Ndt80 in a C. albicans, C. dubliniensis and C. tropicalis during biofilm formation in different media

Project description:The purpose of this experiment was to identify the genes bound by the regulator Ndt80 in C. albicans, C. dubliniensis and C. tropicalis during biofilm formation in different growth media. Ndt80 was tagged with a c-myc epitope in the three species and the immunoprecipitation of the tagged strain was compared to that of an untagged control strain in two different media for each species.

2021-04-14 | GSE161136 | GEO

Chromatin immunoprecipitation of Ndt80-myc at 9 hours in yeast meiosis

Project description:ChIP-chip of Ndt80-myc at 9 hours in yeast meiosis

2009-07-28 | E-MEXP-1779 | biostudies-arrayexpress