Single-cell Gene Expression Analysis Reveals Transcriptional Diversity among Human Spermatogonia

ABSTRACT: Two individual human OCT4-positive spermatogonia were selected for global transcriptional capture using shallow RNA-seq. Successful cDNA synthesis and amplification was confirmed as reads mapped primarily to exons. It has to be noted though, that a comparision of cell to cell data is not possible as coverage of the whole transcriptome was too low, approximately <1.0x coverage. Nonetheless, RNA-seq data provided first information of highly abundant transcripts in individual human spermatogonia. Spermatogonia-specific expression of selected candidate genes with high RPKM values including DDX5, EEF1A1 and TSPY1 was confirmed by immunohistochemistry.

ORGANISM(S): Homo sapiens

PROVIDER: GSE91063 | GEO | 2017/01/15

SECONDARY ACCESSION(S): PRJNA356765

REPOSITORIES: GEO