Project description:The purpose of this experiment was to assess global changes in gene expression pattern in the pancreas from Sox4-/- embryos vs. pancreas from Sox4 wild-type embryos at embryonic day 12.5. [Sox4 gene knock-out (Schilham, M. W. et al. (1996) Defects in cardiac outflow tract formation and pro-B-lymphocyte expansion in mice lacking Sox-4. Nature 380, 711-4.)] Embryonic pancreases from homozygous mutants and their wild-type littermates were harvested. Total RNA was isolated amplified using a linear amplification kit and used to prepare the hybridization samples through indirect labeling. Keywords = Pancreas Sox4 null e12.5 Keywords: parallel sample
Project description:We report bulk RNA-sequencing data for undifferentiated and defined subpopulations of differentiating murine spermatogonia. We have sequenced two replicates each of wild-type and STRA8 knock-out sets of spermatogonia to assess transcriptional changes that occur throughout spermatogonial development prior to meiotic entry.
Project description:Mammalian spermatogenesis is regulated by epigenetic mechanisms that maintain cell type-specific transcriptional programs. The epigenetic regulator BMI1, a PRC1 member, is required for maintaining undifferentiated spermatogonia, but the underlying mechanisms remain unclear. To address this issue, here we performed chromatin immunoprecipitation followed by massive parallel sequencing (ChIP-seq).
Project description:Mammalian spermatogenesis is regulated by epigenetic mechanisms that maintain cell type-specific transcriptional programs. The epigenetic regulator BMI1, a PRC1 member, is required for maintaining undifferentiated spermatogonia, but the underlying mechanisms remain unclear. To address this issue, here we performed RNA-sequencing (RNA-seq) analysis in both wild type and Bmi1 knockdown spermatogonia populations.
Project description:Purpose: The goals of this study are to compare CD1 background spermatogonia veh with NRRA treatment transcriptome profiling (RNA-seq) and to evaluate protocols for in vitro induced meiosis system.Methods: CD1 background spermatogonia mRNA profiles of veh and NRRA treatment were generated by deep sequencing, in duplicate. Methods: CD1 background spermatogonia veh with NRRA treatment were generated by deep sequencing, in duplicate. Conclusions: Our study represents the first detailed analysis of induced meiosis spermatogonia transcriptomes, with biologic replicates, generated by RNA-seq technology.