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Allergen induced gene expression of airway epithelial cells shows a possible role for TNF-α

ABSTRACT: Response to allergen was studied in bronchial epithelial cell line H292. Cells were cultured and subsequently exposed to House dust mite or vessel (saline) Microarray data was analysed using bioinformatics and biostastics. We find a strong response to allergen in epithelial cells Keywords: cellular response to allergen

ORGANISM(S): Homo sapiens

PROVIDER: GSE9151 | GEO | 2007/10/01

SECONDARY ACCESSION(S): PRJNA102701

REPOSITORIES: GEO

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Transcription profiling of mouse bronchial epithelial cell line H292 exposed to allergen reveals a possible role for TNF-I?

Project description:Response to allergen was studied in bronchial epithelial cell line H292. Cells were cultured and subsequently exposed to House dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostastics. We find a strong response to allergen in epithelial cells Experiment Overall Design: Bronchial epithelial cell line was cultured and stimulated with house dust mite extract or diluent alone. Both conditions were performed in triplicate

2008-06-16 | E-GEOD-9151 | biostudies-arrayexpress

Primary nasal epithelium exposed to house dust mite extract shows activated expression in allergics

Project description:Response to allergen was studied in epithelial cells derived from allergic pantients and from healthy controls. Cells were cultured after isolation from a nasal biopsy. Cells were exposed to Housed dust mite or vessel (saline) Microarray data was analysed using bioinformatics and biostaistics. We conclude that a marked difference in basal expression and in response to hous dust mite exists Keywords: cellular response to allergen

2007-11-10 | GSE9150 | GEO

Transcription profiling of human primary nasal epithelium exposed to house dust mite extract shows activated expression in allergics

Project description:Response to allergen was studied in epithelial cells derived from allergic pantients and from healthy controls. Cells were cultured after isolation from a nasal biopsy. Cells were exposed to Housed dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostaistics. We conclude that a marked difference in basal expression and in response to hous dust mite exists Experiment Overall Design: We used 5 monotypic house dust mite allergic patients and 5 non-allergic healthy controls, the cells obtained from the biopsies were cultured in two wells, for two different conditions.

2008-06-16 | E-GEOD-9150 | biostudies-arrayexpress

Allergen Challenge for Evoked Phenotypes in Asthma (the ACE Study)

Project description:This protocol outlines a single-site mechanistic study aiming to investigate long RNAs differentially expressed in the airway epithelium of asthma patients both at baseline and in response to segmental airway allergen challenges. Over approximately 14 days, the study spanned three visits: Visit 1: Comprehensive characterization of participants, encompassing lung function testing, methacholine challenge testing, and allergen skin prick testing. Visit 2: Participants underwent bronchoscopy wherein three procedures were performed a. Epithelial brushings were performed in a segmental airway (baseline sample) b. Diluent (inactive control) was instilled into another segmental airway c. A small dose of allergen was administered into a third segmental airway using standardized cat or dust mite allergen extracts. Visit 3 (24 hours or 7 days post Visit 2): Another bronchoscopy was carried out to collect epithelial brushings in the diluent challenged and allergen challenged segments The collected epithelial brush samples underwent analysis for mRNA expression in the epithelial brushings. The study successfully incorporated a total of 23 subjects, which included 18 asthmatics (with stable or well-controlled conditions), 2 allergic non-asthmatics, and 3 non-allergic non-asthmatics.

2024-03-08 | GSE250241 | GEO

Collaborative Cross founder strain lung miRNA expression after allergen challenge

2015-03-01 | GSE63954 | GEO

Collaborative Cross founder strain lung miRNA expression after allergen challenge

Project description:The goal of this project was to identify strain-dependent expression of miRNAs in lung tissue from Collaborative Cross founder strains that were sensitized and challenged with house dust mite allergen (Der p 1). We analyzed whole lung RNAs from the eight Collaborative Cross founder strains (n=4/strain, all males) using Affymetrix miRNA 2.0 arrays

2015-03-01 | E-GEOD-63954 | biostudies-arrayexpress

Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure

Project description:<h4><strong>BACKGROUND: </strong>Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development.</h4><h4><strong>METHODS: </strong>Calu-3 cells were cultivated in air-liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry.</h4><h4><strong>RESULTS: </strong>We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical-basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L-arginine, L-kynurenine and L-methionine.</h4><h4><strong>CONCLUSION: </strong>This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine-protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence.</h4>

2023-08-22 | MTBLS1755 | MetaboLights

Diesel exhaust and house dust mite allergen lead to common changes in the airway methylome and hydroxymethylome

Project description:Genomewide DNA methylation profiling of saline, diesel exhaust particiles and house dust mite treated human airway epithelial cells. Methylation profiles were generated by the Illumina Infinium MethylationEPIC beadchips.

2018-12-31 | GSE116402 | GEO

Gene Expression Changes Associated With TLR2 Mediated Inhibition of Allergen Specific TH2 Response In Vitro

2012-07-19 | GSE39476 | GEO

Gene Expression Changes Associated With TLR2 Mediated Inhibition of Allergen Specific TH2 Response In Vitro

Project description:Investigation of the gene expression changes associated with TLR2 adjuvant (lipoteichoic acid; LTA) inhibition of allergic TH2 responses in peripheral blood mononuclear cell cultures (derived from house dust mite allergic individuals) stimulated with house dust mite extract. Pooled samples from 5 individuals were analysed by microarray; qPCR validation was carried out in a larger cohort of 23 individuals.

2012-07-18 | E-GEOD-39476 | biostudies-arrayexpress

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