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NIA15k screen. Pax5 +/+, Pax5 -/-, and Rag1-/- pro B cell lines.

ABSTRACT: The transcription factor Pax5 is essential for B cell commitment in the mouse, where it represses lineage-inappropriate gene expression, while simultaneously activating the B cell gene expression program. We have performed a global gene expression screen of wild type and Pax5-deficient pro-B cells in an attempt to identify the crucial Pax5 targets in early B-lymphopoiesis. We have also included Rag1-/- and wild type (+/+) proB cells starved of the cytokine IL-7 for 4 hours as controls. Rag1-/- proB cells are incapable of further differentiation due to an absence of immunoglobulin recombination and IL-7 is the major cytokine regulating proB cell growth. Keywords: comparison of genetically modified cell-lines

ORGANISM(S): Mus musculus

PROVIDER: GSE9345 | GEO | 2007/10/26

SECONDARY ACCESSION(S): PRJNA103033

REPOSITORIES: GEO

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A SIRT7-dependent acetylation switch regulates early B-cell differentiation and lineage commitment through PAX5

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