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Human colon cancer SW480 cells: stably transfected with vector control and LRP16 treated with or without etoposide

ABSTRACT: Transcriptional profiling of human colon cancer SW480 cells comparing control untreated SW480 cells with cells stably transfected with LRP16 treated with or without etoposide (50 μM) for the indicated periods (0,1hour, 3hours).An exploratory microarray analysis was performed with mRNA extracted from clutured SW480 cells transfected with LRP16 or control plasmid that were treated with or without etoposide. Total RNA of colon cancer cells stably transfected with vector control and LRP16 treated with or without etoposide (50 μM) for the indicated periods was isolated and purified using RNeasy Kit (Qiagen, Hilden, Germany). Integrity of RNA was assessed by using an Agilent BioAnalyser 2100 (Agilent Technologies).

ORGANISM(S): Homo sapiens

PROVIDER: GSE93625 | GEO | 2017/09/22

SECONDARY ACCESSION(S): PRJNA361306

REPOSITORIES: GEO

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Homo sapiens

Project description:Human colon cancer SW480 cells: stably transfected with vector control and LRP16 treated with or without etoposide

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LncRNA expression analysis of Clostridium butyricum-induced cell apoptosis in colon cancer SW480 cells

Project description:To compare lncRNAs and mRNAs expression profiles in colon cancer after co-cultured with CB and without CB, we extracted total RNA of colon cancer cell line(SW480) after co-cultured with CB(SW480/CB) and paired control without CB(SW480/ctr), and identified the dysregulated lncRNAs and mRNAs using Agilent Human lncRNAs/mRNAs microarrays.

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Project description:Analysis of differentially expressed genes in colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours. Total RNA obtained from colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours.

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ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29

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Diverse effect of WWOX overexpression in SW480 colon cancer cell line.

Project description:The WWOX gene is a tumor suppressor probably involved in regulation of cell cycle and apoptosis and downregulated in variety of cancer types.However, its role in colon cancerogenesis is unknown. The aim of this study was to characterize how WWOX may be involved in colon cancerogenesis or cancer progression, how it influences the basic cancer cell features and modifies cell expression profile.Our observations suggest that in HT29 colon cancer cell line increased expression of WWOX may result in transition of cancer cells into more normal- like colon epithelium phenotype, on the other hand in SW480 WWOX revealed the well-known tumour suppressor properties. However, as the colon cancer is very heterogeneous disease, obtained discrepancies may reflect the known differences between cell lines and cancerogenesis pathway, which they undergone. SW480 colon cancer cells were stably transfected with WWOX cDNA. SW480 cells transfected with an empty vector served as a control. Total mRNA was isolated to look for gene-expression differences induced by the WWOX overexpression.

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Project description:We transfected GFP and MDM4 into colon cancer cell SW480, and performed transcriptomic analysis.

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Transcription profiling of effected genes by PAD2 and compound 1B8

Project description:To understand the growth inhibition of peptidylarginine deiminase 2 (PAD2) and small molecule 1B8, colon cancer SW480 cells were treated with compound 1B8 for 24 hours, SW480 cells were transfected with humanPAD2 vector for 24 hours. Cells were harvested and total RNA was extracted using TRIZol method followed by Agilent DNA microarray analysis. To profile the effected genes by compound 1B8, SW480 cells were treated with DMSO (control) and 10uM 1B8 in triplicates in 6-well plate, cells were harvested 24 hours later. To compare the effected genes by compound 1B8 and PAD2 overexpression, SW480 cells were transfected with PAD2 and pIRES2-EGFP empty vector in triplicates in 6 well plates, cells were harvested in 24 hours. All the cells were harvested and total RNA was extracted using TRIZol method followed by Agilent DNA microarray analysis.

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Project description:Drug discovery, inhibition of WNT pathway and identification of molecular targets. Prostate EPT1cells were treated with 6-bromoindirubin-3ΓÇÖ-oxime(BIO) or DMSO for 24 hours, colon cancer SW480 cells were treated with compound 3G4 for 24 and 72 hours, SW480 cells were transfected with MED23 knockdown shRNA vector for 48 hours. Cells were harvested and total RNA was extracted using Qiazol method followed by Agilent DNA microarray analysis.

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Human Transcriptome Array 2.0 (HTA) from SW480 colon adenocarcinoma cells transfected with LINC00152 Stealth siRNAs or Stealth Negative Control Medium GC siRNAs

Project description:Whole transcriptome expression levels of SW480 colon adenocarcinoma cells transfected with LINC00152 Stealth siRNAs or Stealth Negative Control Medium GC siRNAs, analyzed by HTA 2.0 microarrays

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Diverse effect of WWOX overexpression in HT29 colon cancer cell line.

Project description:The WWOX gene is a tumor suppressor probably involved in regulation of cell cycle and apoptosis and downregulated in variety of cancer types.However, its role in colon cancerogenesis is unknown. The aim of this study was to characterize how WWOX may be involved in colon cancerogenesis or cancer progression, how it influences the basic cancer cell features and modifies cell expression profile.Our observations suggest that in HT29 colon cancer cell line increased expression of WWOX may result in transition of cancer cells into more normal- like colon epithelium phenotype, on the other hand in SW480 WWOX revealed the well-known tumour suppressor properties. However, as the colon cancer is very heterogeneous disease, obtained discrepancies may reflect the known differences between cell lines and cancerogenesis pathway, which they undergone. HT29 colon cancer cells were stably transfected with WWOX cDNA. HT29 cells transfected with an empty vector served as a control. Total mRNA was isolated to look for gene-expression differences induced by the WWOX overexpression.

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