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Early temporal transcriptome responses of the bovine mid-cycle corpus luteum to prostaglandin F2 alpha indicates cytokine activation

ABSTRACT: In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential for resumption of the estrous cycle and is a target for improving fertility. To deduce the early PGF2α-provoked signaling elicited in the corpus luteum a time-course from 0.5–4 h was performed on cows at mid-cycle. A microarray-determined transcriptome was established and analyzed by Ingenuity Pathway Analysis (IPA). Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of signaling cascades. Classic PGF2α signaling (cyclic adenosine monophosphate, mitogen-activated protein kinases) and cytokine signaling (nuclear factor κB, interleukin) were both predicted by IPA after PGF2α administration. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly changed in each dataset, which also predicted cytokine signaling. Elevated levels of cytokine transcripts after PGF2α and IPA-predicted activation of cytokine pathways demonstrate the importance of inflammatory reactions early in PGF2α -mediated luteolysis.

ORGANISM(S): Bos taurus

PROVIDER: GSE94069 | GEO | 2017/01/26

SECONDARY ACCESSION(S): PRJNA368717

REPOSITORIES: GEO

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