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Effect of PGF2α on the transcriptome of the bovine corpus luteum

ABSTRACT: Effect of PGF2α on the transcriptome of the bovine corpus luteum

PROVIDER: PRJNA896629 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22133900.fastq.gz	Fastqsanger.gz
	SRR22133901.fastq.gz	Fastqsanger.gz
	SRR22133902.fastq.gz	Fastqsanger.gz
	SRR22133903.fastq.gz	Fastqsanger.gz
	SRR22133904.fastq.gz	Fastqsanger.gz

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Effect of lactation and location relative to the corpus luteum on the transcriptome of the bovine oviduct epithelium

Project description:Lactation and associated metabolic stresses during the post-partum period have been shown to impair fertility in dairy cows. The oviduct plays key roles in embryo development and the establishment of pregnancy in cattle. The aim of this study was to investigate the effects of lactation and location relative to the corpus luteum (CL) on the transcriptome of the bovine oviduct epithelium.

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Effect of PGF2α on the transcriptome of the bovine corpus luteum

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Project description:Despite much investigation, mechanisms conferring stage specific responsiveness of the corpus luteum (CL) to prostaglandin F2 (PG) are unknown. The objective of this study was to identify PG- induced changes in transcriptome of bovine CL specific to d 11 ( PG responsive) but not d 4 (PG refractory) CL associated with luteolysis. CL were collected from heifers at 0, 4 and 24 h following PG injection on d 4 and 11 of the estrous cycle (n = 5 animals/treatment) and isolated RNA labeled and hybridized to Affymetrix GeneChip Bovine Genome Arrays. At 4 and 24 h post PG respectively, 221 (d 4) and 661 (d 11) and 248 (d 4) and 1419 (d 11) regulated genes were identified. Thirty Angus crossbred heifers were assigned to one of six treatments (n = 5 per treatment) in a 2 x 3 factorial arrangment, using a completely random design.Corpora lutea were collected at 0 (control), 4 or 24 h following injection of saline (control) or PG on d 4 or d 11 of the estrous cycle. Total RNA was isolated from luteal tissue. The isolated RNA was labeled and hybridized to Affymetrix GeneChip Bovine Genome Arrays.

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Transcriptomic profile of porcine corpus luteum after PPAR gamma ligands treatment

Project description:The corpus luteum plays a critical role in reproduction because it is the primary source of circulating progesterone. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression in the porcine corpus luteum in the mid- and late-luteal phase of the estrous cycle using RNA-seq technology. The corpus luteum slices were incubated in vitro in the presence of PPARγ agonist – pioglitazone and antagonist—T0070907. We identified 40 differentially expressed genes after pioglitazone treatment and 40 after T0070907 treatment in the mid-luteal phase as well as 26 after pioglitazone and 29 after T0070907 treatment in late-luteal phase of the estrous cycle. In addition, we detected differences in genes expression between the mid- and late-luteal phase without treatment (409). These results should become a basis for further studies explaining the mechanism of PPARγ action in the reproductive system in pigs.

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Expression data from bovine corpus luteum treated with equine chorionic gonadotropin (eCG)

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Effect of lactation and location relative to the corpus luteum on the transcriptome of the bovine oviduct epithelium

Project description:Effect of lactation and location relative to the corpus luteum on the transcriptome of the bovine oviduct epithelium

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miRNAs in the porcine corpus luteum

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