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Effect of PGF2α on the transcriptome of the bovine corpus luteum

ABSTRACT: To investigate the rapid effects of PGF2α on the corpus luteum we performed gene expression profiling analysis using data obtained from RNA-seq of controls and treatments at two time points with 4 biological replicates/group .

ORGANISM(S): Bos taurus

PROVIDER: GSE217053 | GEO | 2022/11/04

REPOSITORIES: GEO

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Early temporal transcriptome responses of the bovine mid-cycle corpus luteum to prostaglandin F2 alpha indicates cytokine activation

Project description:In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential for resumption of the estrous cycle and is a target for improving fertility. To deduce the early PGF2α-provoked signaling elicited in the corpus luteum a time-course from 0.5–4 h was performed on cows at mid-cycle. A microarray-determined transcriptome was established and analyzed by Ingenuity Pathway Analysis (IPA). Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of signaling cascades. Classic PGF2α signaling (cyclic adenosine monophosphate, mitogen-activated protein kinases) and cytokine signaling (nuclear factor κB, interleukin) were both predicted by IPA after PGF2α administration. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly changed in each dataset, which also predicted cytokine signaling. Elevated levels of cytokine transcripts after PGF2α and IPA-predicted activation of cytokine pathways demonstrate the importance of inflammatory reactions early in PGF2α -mediated luteolysis.

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Effect of PGF2α on the transcriptome of the bovine corpus luteum

Project description:Effect of PGF2α on the transcriptome of the bovine corpus luteum

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Project description:Despite much investigation, mechanisms conferring stage specific responsiveness of the corpus luteum (CL) to prostaglandin F2 (PG) are unknown. The objective of this study was to identify PG- induced changes in transcriptome of bovine CL specific to d 11 ( PG responsive) but not d 4 (PG refractory) CL associated with luteolysis. CL were collected from heifers at 0, 4 and 24 h following PG injection on d 4 and 11 of the estrous cycle (n = 5 animals/treatment) and isolated RNA labeled and hybridized to Affymetrix GeneChip Bovine Genome Arrays. At 4 and 24 h post PG respectively, 221 (d 4) and 661 (d 11) and 248 (d 4) and 1419 (d 11) regulated genes were identified. Thirty Angus crossbred heifers were assigned to one of six treatments (n = 5 per treatment) in a 2 x 3 factorial arrangment, using a completely random design.Corpora lutea were collected at 0 (control), 4 or 24 h following injection of saline (control) or PG on d 4 or d 11 of the estrous cycle. Total RNA was isolated from luteal tissue. The isolated RNA was labeled and hybridized to Affymetrix GeneChip Bovine Genome Arrays.

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