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Comparison of normal and beta catenin deficient kidney gene expression profiles at E12.5

ABSTRACT: We generated a murine genetic model of beta-catenin deficiency targeted to the ureteric bud cell lineage to study the role of beta-catenin mediated Wnt signaling during ureteric morphogenesis. Keywords: mutation

ORGANISM(S): Mus musculus

PROVIDER: GSE9629 | GEO | 2008/03/25

SECONDARY ACCESSION(S): PRJNA103479

REPOSITORIES: GEO

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Transcription profiling of mouse kideney FROM beta catenin deficient animals vs controls AT E12.5

Project description:We generated a murine genetic model of beta-catenin deficiency targeted to the ureteric bud cell lineage to study the role of beta-catenin mediated Wnt signaling during ureteric morphogenesis. Experiment Overall Design: A comparison was made between triplicate samples of E12.5 kidneys from either normal mice or mice with a b-catenin allele containing LoxP sites flanking exons 2 through 6 crossed to Hoxb7-Cre:Gfp mice

2008-04-04 | E-GEOD-9629 | biostudies-arrayexpress

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Project description:In this study we identify molecules with highly restricted expression patterns during the initial stages of metanephric development, when the ureteric bud has entered the metanephric mesenchyme and initiated branching morphogenesis. Using the Affymetrix Mouse Genome 430 2.0 Array, we compare gene expression patterns in ureteric bud tips, stalks and metanephric mesenchymes from mouse E12.5 embryos. To identify conserved molecular pathways, we also analyze transcriptional profiles in rat E13.5 ureteric buds and metanephric mesenchymes using the Affymetrix Rat Genome U34 Set. Taken together, these data sets help to identify conserved and highly localized transcripts in the metanephric kidney. Keywords = Mus musculus Keywords = Rattus norvegicus Keywords = metanephric mesenchyme Keywords = ureteric bud Keywords = ureteric bud tips Keywords = ureteric bud stalks Keywords = molecular screen for spatially restricted transcripts Keywords: other

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