Project description:Down-regulation of reactive oxygen species build-up in chloroplasts by expression of a plastid-targeted flavodoxin (Fld) delayed localized cell death in tobacco leaves inoculated with the non-host bacterium Xanthomonas campestris pv. vesicatoria (Xcv), while other defensive responses were unaffected. To better understand these effects we compared the transcriptomic alterations caused by Xcv inoculation on leaves of Fld-expressing tobacco plants and their wild-type siblings.
Project description:Here we apply a metabolomics to study the interaction of two co-isolated model organisms, Xanthomonas retroflexus and Paenibacillus amylolyticus. When cultured in close proximity on agar plates Xanthomonas retroflexus induces a clear morphological response from Paenibacillus amylolyticus, leading to enhanced growth of Paenibacillus amylolyticus. Application of 2D spatial metabolomics shows how Xanthomonas retroflexus influences the chemical environment by production specific metabolites.
Project description:Here we apply metabolomics to study the interaction of two co-isolated model organisms, Xanthomonas retroflexus and Paenibacillus amylolyticus. When cultured in close proximity on agar plates Xanthomonas retroflexus induces a clear morphological response from Paenibacillus amylolyticus, leading to enhanced growth of Paenibacillus amylolyticus. Application of 2D spatial metabolomics shows how Xanthomonas retroflexus influences the chemical environment by production specific metabolites.
Project description:Here, we investigated the similarities and differences in the volatile profiles of plants and their holobionts. The VOC profiles of fruits, leaves, stems, and roots from seven plant species, including Turmeric seedlings (Curcuma longa), Artemisia capillaris, Euscaphis japonica, Panax ginseng, Clerodendrum trichotomum, Pyracantha koidzumii, and Japanese cypress (Chamaecyparis obtusa), as well as the VOCs of culturable microbial fractions characterized via GCMS-based metabolomics approaches
Project description:We developed an analysis pipeline that can extract microbial sequences from Spatial Transcriptomic (ST) data and assign taxonomic labels, generating a spatial microbial abundance matrix in addition to the default host expression matrix, enabling simultaneous analysis of host expression and microbial distribution. We called the pipeline Spatial Meta-transcriptome (SMT) and applied it on both human and murine intestinal sections and validated the spatial microbial abundance information with alternative assays. Biological insights were gained from this novel data that that demonstrated host-microbe interaction at various spatial scales. Finally, we tested experimental modification that can increase microbial capture while preserving host spatial expression quality and, by use of positive controls, quantitatively demonstrated the capture efficiency and recall of our methods. This proof of concept work demonstrates the feasibility of Spatial Meta-transcriptomic analysis, and paves the way for further experimental optimization and application.
Project description:Plant pathogenic bacteria disseminate and survive through transmission to and by seeds of hosts and non-hosts plants. To investigate the interaction between xanthomonads and developing seeds of Medicago truncatula, plants at the flower bud stage were spray inoculated until runoff with xanthomonads suspensions. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed on seeds to characterize the molecular dialogue between Xanthomonas campestris pv. campestris in an incompatible situation with M. truncatula seeds and Xanthomonas alfalfae pv. alfalfae in a compatible situation at two developmental time points (16 and 32 days atfter pollination (DAP).
Project description:microRNAs can play a crucial role in stress response in plants, including biotic stress. Some miRNAs are known to respond to bacterial infection. This work has addressed the role of miRNAs in Manihot esculenta (cassava)-Xanthomonas axonopodis pv. manihotis (Xam) interaction. Illumina sequencing was used for analyzing small RNA libraries from cassava tissue infected and non-infected with Xam. Cassava variety MBRA685 (resistant to Xam-CIO151) Six-week-old plants were inoculated with 36h-old cultures of the aggressive Xanthomonas axonopodis pv. manihotis strain CIO151 in both leaves and stems.
Project description:Transcription profiling of the DSF regulon in Xanthomonas oryzae pv. oryzae (Xoo) using wild type and the rpfF mutant. Cell-cell signaling mediated by the quorum sensing molecule known as Diffusible Signaling factor (DSF) is required for virulence of Xanthomonas group of plant pathogens. DSF in different Xanthomonas and the closely related plant pathogen Xylella fastidiosa regulates diverse traits in a strain specific manner. The transcriptional profiling performed in this study is to elucidate the traits regulated by DSF from the Indian isolate of Xanthomonas oryzae pv. oryzae, which exhibits traits very different from other Xanthomonas group of plant pathogen. In this study, transcription analysis was done between a wild type Xanthomonas oryzae pv. oryzae strain and an isogenic strain that has a mutation in the DSF biosynthetic gene rpfF.