Project description:This entry contains the proteomic files from a data set of RNAs, proteins, metabolites and lipids analyzed from the same staged samples of S. cerevisiae cells across the cell cycle. Actively growing (un-arrested) diploid cells were collected by elutriation as 8 distinct size pools across the cell cycle in triplicate. Yeast in these 24 aliquots were lysed and soluble and insoluble proteins were prepared for shotgun LC-MS/MS mass spectrometry analysis.
Project description:For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells.
Project description:We use Saccharomyces cerevisiae to perform absolute quantitative multi-omics analysis to map interactions of different cellular processes during the yeast cell cycle.
Project description:We use Saccharomyces cerevisiae, grown on glucose and synchronized with CDC15-2, to map interactions of different cellular processes during the yeast cell cycle.
Project description:We use Saccharomyces cerevisiae grown on ethanol to perform absolute quantitative multi-omics analysis to map interactions of different cellular processes during the yeast cell cycle.
Project description:Dynamins are large multidomain GTPases involved in membrane scission. We have investigated the transcriptional consequences of deleting Vps1, a DNM2 homologue, in the yeast Saccharomyces cerevisiae. Interestingly, the vps1∆ mutation causes strong transcriptional defects also in yeast. Data suggest that DNM2 and Vps1 play an essential regulatory role connecting several membrane-related processes including endocytosis, lipid homeostasis and different signal transduction pathways.
Project description:Wild type and sgs1 null yeast were grown under DNA damaging (with MMS) conditions or without treatment to log phase and their transcriptional profiles compared . The human aging diseases Werner and Bloom syndromes are a result of mutation of the WRN and BLM genes, respectively. The SGS1 gene of Saccharomyces cerevisiae is homologous to the human WRN and BLM genes of the RecQ DNA helicase family. Deletion of SGS1 results in accelerated yeast aging and a reduction in life span as well as cell cycle arrest. We demonstrate that SGS1 deletion, DNA damage, and stress show similar transcriptional responses in yeast. Our comparative analysis of the genome-wide expression response of SGS1 deletion, stress and DNA damage indicates parallel transcriptional responses to cellular insult and aging in yeast. Keywords: other