GNPS - SiTrap versus STrap comparison
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Velos
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Alexandre Zougman
PROVIDER: MSV000084406 | GNPS | Wed Oct 02 04:44:00 BST 2019
SECONDARY ACCESSION(S): PXD015678
REPOSITORIES: GNPS
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Project description:These are supporting RAW files for the 'Detergent-free simultaneous sample preparation method for proteomics and metabolomics' SiTrap method manuscript.
MDA-MB-231 cells were processed either by SiTrap methodology using SiTrap cellulose tips and two different extraction solutions or by STrap method using STrap quartz tips and SDS for extraction. 30 ug of protein was digested with trypsin in six replicates in each case. The peptides were eluted from the tips and concentrated for proteomics analysis.
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Project description:These are supporting RAW files for 'Detergent-free simultaneous sample preparation method for proteomics and metabolomics' SiTrap method manuscript.
Frozen renal tissue from three matched clear cell renal carcinoma (G2 pT3a, G2 pT1b, G1 pT2) /adjacent normal sample pairs were obtained from The Leeds Multidisciplinary Research Tissue Bank. Approximately 1 cm2 sections with a thickness of 10 um were lysed and processed according to SiTrap protocol using SiTrap cellulose tips. The SiTrap load was normalized by protein concentration. 50 ug of protein was loaded. The collected flow-through fraction, devoid of proteins, was dried down for targeted metabolomics analysis. The captured protein fraction was reduced and alkylated in situ and digested with trypsin according to SiTrap protocol. The resulting peptides were concentrated for proteomics analysis.
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Project description:These are supporting RAW files for 'Detergent-free simultaneous sample preparation method for proteomics and metabolomics' SiTrap method manuscript.
Frozen renal tissue from three matched clear cell renal carcinoma (G2 pT3a, G2 pT1b, G1 pT2) /adjacent normal sample pairs were obtained from The Leeds Multidisciplinary Research Tissue Bank. Approximately 1 cm2 sections with a thickness of 10 um were lysed and processed according to SiTrap protocol using SiTrap cellulose tips. The SiTrap load was normalized by protein concentration. 50 ug of protein was loaded. The collected flow-through fraction, devoid of proteins, was dried down for targeted metabolomics analysis. The captured protein fraction was reduced and alkylated in situ and digested with trypsin according to SiTrap protocol. The resulting peptides were concentrated for proteomics analysis.
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