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SiTrap proteomics profiling of renal normal/tumor tissue pairs

ABSTRACT: These are supporting RAW files for 'Detergent-free simultaneous sample preparation method for proteomics and metabolomics' SiTrap method manuscript. Frozen renal tissue from three matched clear cell renal carcinoma (G2 pT3a, G2 pT1b, G1 pT2) /adjacent normal sample pairs were obtained from The Leeds Multidisciplinary Research Tissue Bank. Approximately 1 cm2 sections with a thickness of 10 um were lysed and processed according to SiTrap protocol using SiTrap cellulose tips. The SiTrap load was normalized by protein concentration. 50 ug of protein was loaded. The collected flow-through fraction, devoid of proteins, was dried down for targeted metabolomics analysis. The captured protein fraction was reduced and alkylated in situ and digested with trypsin according to SiTrap protocol. The resulting peptides were concentrated for proteomics analysis.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Alexandre Zougman

PROVIDER: MSV000084405 | MassIVE | Wed Oct 02 03:50:00 BST 2019

SECONDARY ACCESSION(S): PXD015677

REPOSITORIES: MassIVE

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